Pak Ilona, Askarov Meirambek, Klyuyev Dmitriy, Tak Min Sungh, Batenova Ulpan, Yeskermessov Dauren, Kamyshanskiy Yevgeniy
Plastic Surgery Department, Karaganda State Medical, Karaganda, Kazakhstan.
Life Science Institute, Karaganda State Medical, Karaganda, Kazakhstan.
Front Bioeng Biotechnol. 2025 Apr 11;13:1545419. doi: 10.3389/fbioe.2025.1545419. eCollection 2025.
Lipofilling is gaining in popularity daily as a method of replenishing the volume of almost any part of the human body. However, the use of adipose tissue as a filler has its limitations in the long term, in the form of a low survival rate of the fat graft because of fibrotic replacement and fat cell apoptosis. The aim of this study was a comparative morphological assessment of fat autograft survival in the groups undergoing a standard lipofilling protocol and the pre-implantation treatment of the implantation area with platelet-rich plasma.
Twenty-four male Californian rabbits that had undergone hypodermic implantation of a fat autograft in the area of the auricle were used in the study. All cases were classified into three groups depending on the method of platelet-rich plasma treatment. After 3 months (90 days) of exposure, macroscopic and histological examinations of the fat autograft were conducted.
The volume and histological normality of the fat autograft were statistically significantly preserved in the group with preoperative treatment of the implant area and intraoperative treatment of the autograft compared to the group without it and with intraoperative treatment alone.
We have demonstrated that the pre-implantation use of platelet-rich plasma significantly improves the standard intraoperative technique and increases the survival rate of the fat autograft by enhancing angiogenesis, with a decreased degree of hypoxic-ischemic, fibrotic, and inflammatory damage in the implant area.
The effect of improved preservation of the morphological pattern of the fat implant during preoperative treatment may be due to a favorable preoperative locoregional stromal-vascular microenvironment with an active perfusion and diffusion potential of the stromal skeleton.
脂肪填充作为一种补充人体几乎任何部位容积的方法，日益受到欢迎。然而，将脂肪组织用作填充物存在长期局限性，表现为由于纤维化替代和脂肪细胞凋亡导致脂肪移植物存活率较低。本研究的目的是对接受标准脂肪填充方案的组和用富血小板血浆对植入区域进行植入前处理的组中的自体脂肪移植物存活情况进行比较形态学评估。
本研究使用了24只在耳廓区域进行了自体脂肪皮下植入的雄性加利福尼亚兔。根据富血小板血浆处理方法，所有病例分为三组。暴露3个月（90天）后，对自体脂肪移植物进行宏观和组织学检查。
与未进行植入区域术前处理仅进行术中处理的组相比，进行了植入区域术前处理和自体脂肪移植物术中处理的组中，自体脂肪移植物的体积和组织学正常性在统计学上得到了显著保留。
我们已经证明，植入前使用富血小板血浆可显著改善标准术中技术，并通过增强血管生成提高自体脂肪移植物的存活率，同时降低植入区域的缺氧缺血、纤维化和炎症损伤程度。
术前处理期间脂肪植入物形态模式保存改善的效果可能归因于术前局部区域具有有利的基质血管微环境，其基质骨架具有活跃的灌注和扩散潜能。
