富血小板血浆是否应在脂肪移植中被激活？一项动物研究。

Should platelet-rich plasma be activated in fat grafts? An animal study.

机构信息

Department of Plastic, Reconstructive and Aesthetic Surgery, Henri Mondor Hospital, 51 avenue de Lattre de Tassigny 94000, Creteil, France; UPEC, University Paris Est Creteil, Val de Marne, France; INSERM U955, Equipe 12, Creteil, France.

出版信息

J Plast Reconstr Aesthet Surg. 2018 May;71(5):681-690. doi: 10.1016/j.bjps.2018.01.005. Epub 2018 Feb 2.

DOI:10.1016/j.bjps.2018.01.005

PMID:29477267

Abstract

BACKGROUND

The adjunction of platelet-rich plasma with graft fat has been the subject of a few clinical trials which have demonstrated its value in adipocyte survival. The aim of this study was to assess the different efficacies between activated and non-activated PRP on adipose cells in vitro and for adipose tissue graft survival in vivo.

METHODS

The in vitro study assessed the effects of PRP on both the proliferation and adipocyte differentiation of adipose cells. For the in vivo study, 8 nude rats received 3 human fat injections as follows: 0.8 mL of fat + 0.2 mL of normal saline; 0.8 mL of fat + 0.2 mL of non-activated PRP; and 0.8 mL of fat + 0.2 mL of PRP activated with calcium chloride (CaCl). The quantitative assessment of adipocyte survival was implemented after 3 months using histomorphometric analysis. Histological and immunohistochemical analysis were also performed to evaluate angiogenesis, inflammation and quality of adipocytes in the grafted tissue.

RESULTS

We showed that activated PRP stimulated, in vitro, proliferation and differentiation of adipose cells. In vivo experiments indicated that CaCl-activated PRP was more efficient than non-activated to prolong the survival of fat grafts in nude rats. The mean percentage areas occupied by viable adipocytes in the PRP-free group, non-activated PRP group and activated PRP group were 13%, 14% and 24% (p = 0.05%), respectively. Histological and immunohistochemical analysis revealed protective effect of activated PRP on inflammation and adipocyte death.

CONCLUSION

This study showed that activation by CaCl improves the beneficial effects of PRP for fat graft maintenance.

摘要

背景

富含血小板的血浆与脂肪移植物的联合应用已成为少数临床试验的主题，这些试验证明了其在脂肪细胞存活方面的价值。本研究旨在评估激活和非激活 PRP 在体外对脂肪细胞和体内脂肪组织移植物存活的不同疗效。

方法

体外研究评估了 PRP 对脂肪细胞增殖和脂肪细胞分化的影响。对于体内研究，8 只裸鼠接受了 3 个人体脂肪注射，如下所示：0.8mL 脂肪+0.2mL 生理盐水；0.8mL 脂肪+0.2mL 非激活 PRP；和 0.8mL 脂肪+0.2mL 用氯化钙（CaCl）激活的 PRP。在 3 个月后，通过组织形态计量分析对脂肪细胞存活的定量评估。还进行了组织学和免疫组织化学分析，以评估移植物组织中的血管生成、炎症和脂肪细胞质量。

结果

我们表明，激活的 PRP 刺激了体外脂肪细胞的增殖和分化。体内实验表明，与非激活 PRP 相比，CaCl 激活的 PRP 更有效地延长裸鼠脂肪移植物的存活。在无 PRP 组、非激活 PRP 组和激活 PRP 组中，存活脂肪细胞的平均面积百分比分别为 13%、14%和 24%（p=0.05%）。组织学和免疫组织化学分析显示，激活 PRP 对炎症和脂肪细胞死亡具有保护作用。