Department of Plastic, Reconstructive & Aesthetic Surgery, University Hospitals of Geneva and Faculty of Medicine, 4 Rue Gabrielle-Perret-Gentil, 1211 Geneva 14, University of Geneva, Switzerland.
MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211 Geneva 14, Switzerland.
J Plast Reconstr Aesthet Surg. 2019 Apr;72(4):669-675. doi: 10.1016/j.bjps.2018.12.039. Epub 2019 Jan 7.
The adjunction of autologous platelet-rich plasma (PRP) is emerging as a promising approach to enhance the long-term survival of fat grafting, but there are still insufficient data on its efficacy. The aim of this in vivo study was to assess the effect of the addition of non-activated PRP on fat graft outcome.
Human adipose tissue mixed with 20% of non-activated PRP was injected under the scalp skin of nude Balb/cAnNRj mice and compared to grafted fat mixed with 20% of saline. The fat graft volume was analyzed by a computed tomography scan until day 90 and immunohistochemistry was then performed to assess adipocyte viability and graft revascularization.
At day 90, the volume of fat graft was not enhanced by PRP compared to the saline control group. However, immunohistochemistry showed that PRP significantly increased the fat graft area occupied by intact adipocytes compared to the saline group (72.66% vs. 60.78%, respectively; p < 0.05). Vascularity was also significantly higher in the PRP group compared to the control group (6695 vs. 4244 CD31 cells/µm, respectively; p < 0.05).
The adjunction of non-activated-PRP to fat grafts significantly increased adipocyte viability and tissue vascularity. However, in contrast to other studies adding activated-PRP, non-activated-PRP did not increase residual fat graft volume until day 90. Further studies are therefore needed to understand whether PRP has a positive effect on fat graft volume. As 3D computed tomography scan is a reproducible and precise technique, it should be used to analyze fat graft volume changes over time.
自体富血小板血浆（PRP）的添加作为一种增强脂肪移植长期存活率的有前途的方法正在出现，但关于其疗效的数据仍然不足。本体内研究旨在评估添加非激活 PRP 对脂肪移植结果的影响。
将与人脂肪组织混合的 20%非激活 PRP 注入裸鼠 Balb/cAnNRj 头皮下皮肤，并与与 20%盐水混合的移植脂肪进行比较。通过计算机断层扫描分析脂肪移植体积，直到第 90 天，然后进行免疫组织化学检查以评估脂肪细胞活力和移植物再血管化。
与盐水对照组相比，PRP 并未增加第 90 天的脂肪移植体积。然而，免疫组织化学显示 PRP 显著增加了完整脂肪细胞占据的脂肪移植面积，与盐水组相比（分别为 72.66%和 60.78%；p<0.05）。PRP 组的血管化也明显高于对照组（分别为 6695 和 4244 CD31 细胞/µm；p<0.05）。
将非激活 PRP 添加到脂肪移植中可显著增加脂肪细胞活力和组织血管化。然而，与添加激活 PRP 的其他研究相反，非激活 PRP 直到第 90 天才增加残留脂肪移植体积。因此，需要进一步研究以了解 PRP 是否对脂肪移植体积有积极影响。由于 3D 计算机断层扫描是一种可重复且精确的技术，因此应使用该技术分析随时间推移的脂肪移植体积变化。
