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耦合毛细管电泳与偏移进样电位高分辨率离子迁移谱仪

Coupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer.

作者信息

Welters Klaus, Thoben Christian, Raddatz Christian-Robert, Schlottmann Florian, Zimmermann Stefan, Belder Detlev

机构信息

Institute of Analytical Chemistry, Leipzig University, Leipzig, Germany.

Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz University Hannover, Hannover, Germany.

出版信息

Electrophoresis. 2025 Jun;46(11-12):694-701. doi: 10.1002/elps.8147. Epub 2025 Apr 28.

DOI:10.1002/elps.8147
PMID:40292850
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12366286/
Abstract

We present the coupling of capillary electrophoresis to a custom-built high-resolution ion mobility spectrometer (IMS). This system integrates a shifted inlet potential IMS configuration with a customised nanoflow ESI sheath interface. It enables the rapid analysis of quaternary ammonium compounds (QACs) and their impurities in real-world samples. It allowed the detection of six non-chromophoric compounds in about 3 min. The assignment of the IMS signals to compounds was supported by matching experimentally determined collision cross-section (CCS) values with predicted values. The system achieved a detection limit in the single-digit picogram range with IMS resolutions of over 80.

摘要

我们展示了毛细管电泳与定制的高分辨率离子迁移谱仪（IMS）的联用。该系统将偏移入口电位的IMS配置与定制的纳流电喷雾电离（ESI）鞘流接口相结合。它能够对实际样品中的季铵化合物（QACs）及其杂质进行快速分析。在约3分钟内可检测到六种非发色化合物。通过将实验测定的碰撞截面（CCS）值与预测值相匹配，支持了将IMS信号分配给化合物。该系统在IMS分辨率超过80的情况下，实现了个位数皮克范围内的检测限。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/e4c4eb7f897c/ELPS-46--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/6beab4df399c/ELPS-46--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/924f50335701/ELPS-46--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/673539e6dbca/ELPS-46--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/11674c1eee11/ELPS-46--g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/ff0ca04f5f03/ELPS-46--g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/e4c4eb7f897c/ELPS-46--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/6beab4df399c/ELPS-46--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/924f50335701/ELPS-46--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/673539e6dbca/ELPS-46--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/11674c1eee11/ELPS-46--g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/ff0ca04f5f03/ELPS-46--g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e0/12366286/e4c4eb7f897c/ELPS-46--g004.jpg

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A novel coupling technique based on thermal desorption gas chromatography with mass spectrometry and ion mobility spectrometry for breath analysis.
一种基于热脱附气相色谱-质谱和离子淌度谱联用的新型呼吸分析耦合技术。
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Ion mobility in gas and liquid phases: How much orthogonality is obtained in capillary electrophoresis-ion mobility-mass spectrometry?气相和液相中的离子迁移：毛细管电泳-离子迁移-质谱中获得了多少正交性？
Electrophoresis. 2024 Apr;45(7-8):735-742. doi: 10.1002/elps.202300210. Epub 2023 Dec 12.
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METLIN-CCS: an ion mobility spectrometry collision cross section database.METLIN-CCS：一个离子淌度谱碰撞截面数据库。
Nat Methods. 2023 Dec;20(12):1836-1837. doi: 10.1038/s41592-023-02078-5.
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