Edalat Fahime, Gholamzad Amir, Ghoreshi Zohreh-Al-Sadat, Dalfardi Mohammad, Golkar Ahmad, Behboudi Emad, Arefinia Nasir
Autophagy Research Center, Shiraz University of Medical Science, Shiraz, Iran.
Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Virus Genes. 2025 Apr 28. doi: 10.1007/s11262-025-02159-x.
This study aimed to investigate the prevalence and phylogenetic characteristics of Human T-cell lymphotropic virus type 1 (HTLV-1) among blood donors in Jiroft Province, southeast Iran, a region previously under-studied regarding this virus. A total of 405 blood donor samples were collected from six cities within Jiroft Province. Serum samples were screened for HTLV-1 antibodies using the ELISA method, while peripheral blood mononuclear cells (PBMCs) were analyzed via PCR targeting the long terminal repeat (LTR) and TAX regions of the virus. The study identified 6 out of 405 blood donors (1.5%) as positive for HTLV-1. Prevalence was higher among females (1.6%) compared to males (1.2%), with the age group of 46-64 years showing the highest positivity rate (4%). Phylogenetic analysis revealed that the LTR sequences of HTLV-1 in Jiroft were comparable to those circulating in Mashhad Province, with nine single-nucleotide polymorphisms (SNPs) identified in the LTR region of the isolates. The findings highlight the necessity for routine HTLV-1 screening among blood donors in Jiroft to ensure blood safety and mitigate the risk of transmission through transfusions. This study provides essential baseline data on HTLV-1 prevalence in Jiroft and contributes to the understanding of its genetic diversity, emphasizing the need for further research in this area.
本研究旨在调查伊朗东南部吉罗夫特省献血者中1型人类嗜T细胞病毒（HTLV-1）的流行情况及其系统发育特征，该地区此前在这种病毒方面的研究较少。从吉罗夫特省的六个城市共采集了405份献血者样本。使用酶联免疫吸附测定（ELISA）方法对血清样本进行HTLV-1抗体筛查，同时通过针对病毒长末端重复序列（LTR）和TAX区域的聚合酶链反应（PCR）分析外周血单个核细胞（PBMC）。该研究确定405名献血者中有6人（1.5%）HTLV-1呈阳性。女性（1.6%）的患病率高于男性（1.2%），46 - 64岁年龄组的阳性率最高（4%）。系统发育分析表明，吉罗夫特HTLV-1的LTR序列与在马什哈德省流行的序列相当，在分离株的LTR区域鉴定出9个单核苷酸多态性（SNP）。这些发现凸显了在吉罗夫特对献血者进行常规HTLV-1筛查以确保血液安全并降低输血传播风险的必要性。本研究提供了吉罗夫特HTLV-1流行情况的重要基线数据，并有助于了解其遗传多样性，强调了该领域进一步研究的必要性。
