Li Qi, Peng Zi-Qi, Liu Wen-Ling, Luo Zhen-Hao, Li Shuai, Li Wen-Jun, Dong Lei
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
School of Life Sciences, Conservation and Utilization Laboratory of Mountain Characteristic Resources in Guangdong Province, Jiaying University, Meizhou, PR China.
Int J Syst Evol Microbiol. 2025 Apr;75(4). doi: 10.1099/ijsem.0.006771.
A Gram-stain-negative, aerobic, rod-shaped bacterial strain, SYSU D60014, was isolated from a sandy soil sample collected from the Gurbantunggut Desert in Xinjiang, PR China. Colonies of SYSU D60014 were pink-coloured, crystalline, irregular edge with a rough surface. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain SYSU D60014 belonged to the family , formed a distinct lineage and showed 92.5-91.0% similarity to closely related strains. Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between strain SYSU D60014 and members of its related species were 17.2-19.6%, 70.8-74.4% and 48.9-64.5%, respectively. The complete genome of strain SYSU D60014 was 5,100,926 bp with a DNA G+C content of 65.1%. Cells were oxidase-positive and catalase-negative. The strain could grow at 28-40 °C (optimum, 37 °C), pH 5.0-8.0 (optimum, pH 7.0), and in the presence of up to 3% NaCl (optimum, 1-1.5%, w/v) on R2A. The predominant menaquinone was Q-10. The main polar lipids were phosphatidylethanolamine, phosphatidylmethyl ethanolamine, diphosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, unidentified phospholipid, four unidentified polar lipids and two unidentified phosphoglycolipids. Major cellular fatty acids were C cyclo 8, summed feature 8 (C c and/or C c) and C. Based on the results obtained through genotypic and phenotypic analyses, we propose that strain SYSU D60014 represents a novel species and genus within the family , for which we propose the name gen. nov., sp. nov. (type strain SYSU D60014=NBRC 112951=CPCC 101030).
从中国新疆古尔班通古特沙漠采集的一份沙质土壤样本中分离出了一株革兰氏阴性、需氧、杆状细菌菌株SYSU D60014。SYSU D60014的菌落呈粉红色、晶体状，边缘不规则，表面粗糙。基于16S rRNA基因和全基因组序列的系统发育分析表明，菌株SYSU D60014属于[具体科名未给出]科，形成了一个独特的谱系，与亲缘关系密切的菌株显示出92.5 - 91.0%的相似性。菌株SYSU D60014与其相关物种成员之间的数字DNA - DNA杂交、平均核苷酸同一性和平均氨基酸同一性值分别为17.2 - 19.6%、70.8 - 74.4%和48.9 - 64.5%。菌株SYSU D60014的完整基因组为5,100,926 bp，DNA G + C含量为65.1%。细胞氧化酶呈阳性，过氧化氢酶呈阴性。该菌株可在28 - 40°C（最适温度37°C）、pH 5.0 - 8.0（最适pH 7.0）以及R2A培养基上存在高达3% NaCl（最适浓度1 - 1.5%，w/v）的条件下生长。主要的甲基萘醌为Q - 10。主要的极性脂为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、二磷脂酰甘油、未鉴定的氨基磷脂、未鉴定的氨基脂、未鉴定的磷脂、四种未鉴定的极性脂和两种未鉴定的磷酸糖脂。主要的细胞脂肪酸为C环8、总和特征8（C c和/或C c）以及C。基于通过基因型和表型分析获得的结果，我们提议菌株SYSU D60014代表[具体科名未给出]科内的一个新物种和新属，并为此提议命名为[新属名]属，新种（模式菌株SYSU D60014 = NBRC 112951 = CPCC 101030）。
