Lessing J B, Brenner S H, Schoenfeld C, Goldsmith L T, Amelar R D, Dubin L, Weiss G
Fertil Steril. 1985 Sep;44(3):406-9. doi: 10.1016/s0015-0282(16)48868-0.
In an attempt to improve the motility of thawed spermatozoa after 1 week of its being frozen, we treated ten normal semen samples with 16.7 nmol relaxin and 6 mmol caffeine. Motility and grade of forward progression were examined after thawing. Caffeine significantly improved motility of spermatozoa. Relaxin, when added to semen at the time of thawing, improved motility and grade of forward progression significantly. This effect was not seen if relaxin was added before freezing. These results suggest that the biologic activity of relaxin is adversely affected by freezing and thawing in seminal plasma. These observations may have clinical importance for the improvement of motility of frozen spermatozoan samples at the time of artificial insemination and for the possible improvement of pregnancy rates.
为了提高冷冻1周后的解冻精子的活力，我们用16.7纳摩尔的松弛素和6毫摩尔的咖啡因处理了10份正常精液样本。解冻后检查精子活力和向前运动等级。咖啡因显著提高了精子的活力。解冻时向精液中添加松弛素可显著提高精子活力和向前运动等级。如果在冷冻前添加松弛素，则看不到这种效果。这些结果表明，精液中的冷冻和解冻会对松弛素的生物活性产生不利影响。这些观察结果对于人工授精时提高冷冻精子样本的活力以及可能提高妊娠率可能具有临床意义。
