Colon J M, Ginsburg F, Lessing J B, Schoenfeld C, Goldsmith L T, Amelar R D, Dubin L, Weiss G
Fertil Steril. 1986 Dec;46(6):1133-9.
Relaxin and prostaglandin E2 (PGE2) are present in human semen and have been shown to affect sperm motility. The authors further examined the effects of porcine relaxin and PGE2 on the motility of human spermatozoa. A dose-response study revealed that PGE2 at a concentration of 25 micrograms/ml is most effective in improving the motility of washed human sperm. Relaxin (100 ng/ml), PGE2 (25 micrograms/ml), or the two combined have no effect on the motility of spermatozoa in fresh, normal semen, suggesting that the constituents of fresh semen are optimal for motility. Relaxin and PGE2 individually improve the motility of washed spermatozoa. However, relaxin, but not PGE2, improves the motility of sperm in semen incubated at 37 degrees C for 5 hours (aged). In contrast to the individual substances, a combination of relaxin + PGE2 has no effect on the motility of washed spermatozoa or aged spermatozoa, suggesting that these two substances antagonize each other's actions on sperm motility. The presence of both relaxin and PGE2 in seminal plasma with normal motility spermatozoa suggests that other factors in seminal plasma regulate the effects of these substances on sperm motility.
松弛素和前列腺素E2（PGE2）存在于人类精液中，并且已被证明会影响精子活力。作者进一步研究了猪松弛素和PGE2对人类精子活力的影响。一项剂量反应研究表明，浓度为25微克/毫升的PGE2在改善洗涤后的人类精子活力方面最有效。松弛素（100纳克/毫升）、PGE2（25微克/毫升）或两者联合使用对新鲜正常精液中的精子活力没有影响，这表明新鲜精液的成分对精子活力而言是最佳的。松弛素和PGE2单独使用均可提高洗涤后精子的活力。然而，松弛素而非PGE2可提高在37摄氏度下孵育5小时（老化）的精液中精子的活力。与单独使用这些物质不同，松弛素+PGE2组合对洗涤后精子或老化精子的活力没有影响，这表明这两种物质在精子活力方面相互拮抗对方的作用。在具有正常活力精子的精浆中同时存在松弛素和PGE2，这表明精浆中的其他因素调节了这些物质对精子活力的影响。
