Barkay J, Bartoov B, Ben-Ezra S, Langsam J, Feldman E, Gordon S, Zuckerman H
Fertil Steril. 1984 Jun;41(6):913-8. doi: 10.1016/s0015-0282(16)47907-0.
After publication in the literature that in vitro caffeine treatment causes damage of the normal shape of the sperm head and thereby decreases fertilizing capacity, we carried out a clinical and electron microscopic study to determine the influence of caffeine on the fertilizing capacity and sperm cell morphology. Sixty women (with infertile husbands) underwent artificial insemination by donor with frozen/thawed semen over a period of 12 months, using randomized addition of caffeine in alternate months. Fourteen women became pregnant during the 6 months they received caffeine-treated semen, whereas only 7 pregnancies occurred during the 6 months the women received semen without caffeine. Scanning electron microscopic examinations of fresh proven donor semen showed no morphologic changes caused by the in vitro caffeine treatment. However, quantitative morphologic analysis of the frozen/thawed semen was unsatisfactory because of the freezing technique and the masking effect of the protective medium. It is concluded that in vitro caffeine treatment of fertile donor semen does not damage the spermatozoa; furthermore, it seems to improve the fertilizing capacity.
在文献发表指出体外咖啡因处理会导致精子头部正常形态受损从而降低受精能力之后，我们开展了一项临床和电子显微镜研究，以确定咖啡因对受精能力和精子细胞形态的影响。60名女性（其丈夫不育）在12个月的时间里接受了供体冷冻/解冻精液人工授精，交替月份随机添加咖啡因。14名女性在接受咖啡因处理精液的6个月内怀孕，而在接受无咖啡因精液的6个月内仅有7例怀孕。对新鲜的经证实的供体精液进行扫描电子显微镜检查显示，体外咖啡因处理未引起形态学变化。然而，由于冷冻技术和保护介质的掩盖作用，对冷冻/解冻精液的定量形态学分析并不理想。得出的结论是，对可育供体精液进行体外咖啡因处理不会损害精子；此外，似乎还能提高受精能力。
