Barbeck Mike, Huebner Kerstin, Burkhardt Kim, Andreeva Tonya, Krastev Rumen, Schnettler Reiner, Stojanovic Sanja, Najman Stevo, Jung Ole, Schneider-Stock Regine
Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany;
Experimental Tumor Pathology, Institute of Pathology, Universitätsklinikum Erlangen, FAU Erlangen-Nürnberg, Erlangen, Germany.
In Vivo. 2025 May-Jun;39(3):1740-1750. doi: 10.21873/invivo.13977.
BACKGROUND/AIM: The present study aimed to compare the tissue responses to biomaterials in the chick chorioallantoic membrane (CAM) model with those from the subcutaneous implantation model in rats at an early time point. It was especially investigated whether histopathological scoring according to DIN EN ISO 10993-6 is also possible after biomaterial implantation using the CAM model and to what extent the values differ from the data obtained from small animal experiments.
Implantation of a xenogeneic bone substitute using the CAM model for 24 h and subcutaneous implantation model in rats up to 10 days post implantation were conducted. Standardized histological and histopathological methods were used to apply for histopathological scoring according to DIN EN ISO 10993-6.
The histological analysis as well as the histo-pathological scoring revealed that the tissue responses to the xenogeneic bone substitute were completely comparable in both organisms with no visible or statistical differences.
We suggest that bioincompatible biomaterials can already be sorted out in the context of THE preclinical test phase. Such pre-testing before the required small animal tests might clearly contribute to the 3R-concept to reduce the number of animals (REDUCE).
背景/目的：本研究旨在比较鸡胚绒毛尿囊膜（CAM）模型和大鼠皮下植入模型在早期时间点对生物材料的组织反应。特别研究了使用CAM模型植入生物材料后，是否也可以根据DIN EN ISO 10993-6进行组织病理学评分，以及这些值与从小型动物实验获得的数据有多大差异。
使用CAM模型植入异种骨替代物24小时，并在大鼠皮下植入模型中观察至植入后10天。采用标准化的组织学和组织病理学方法，根据DIN EN ISO 10993-6进行组织病理学评分。
组织学分析和组织病理学评分显示，两种生物对异种骨替代物的组织反应完全可比，无明显或统计学差异。
我们建议在临床前测试阶段就可以筛选出生物不相容的生物材料。在进行所需的小型动物试验之前进行此类预测试，可能会明显有助于3R概念，即减少动物数量（REDUCE）。
