锶铜掺杂生物玻璃的免疫反应分析。
Analysis of the Immune Response to Strontium- and Copper-doped Bioglass.
机构信息
Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany;
BerlinAnalytix GmbH, Berlin, Germany.
出版信息
In Vivo. 2022 Sep-Oct;36(5):2149-2165. doi: 10.21873/invivo.12941.
BACKGROUND
Bioglass is a highly adoptable bone substitute material which can be combined with so-called therapeutic ions. However, knowledge is poor regarding the influence of therapeutic ions on immune reactions and associated bone healing. Thus, the aim of this work was to investigate the influence of strontium- and copper-doped bioglass on the induction of M1 and M2 macrophages, as well as vascularization.
MATERIALS AND METHODS
Two types of alkali glass were produced based on ICIE16 bioglass via the melt-quench method with the addition of 5 wt% copper or strontium (ICIE16-Cu and ICIE16-Sr). Pure ICIE16 and 45S5 bioglass were used as control materials. The ion release and chemical composition of the bioglass were investigated, and an in vivo experiment was subcutaneously performed on Sprague-Dawley rats.
RESULTS
Scanning electron microscopy revealed significant differences in the surface morphology of the bioglass materials. Energy dispersive X-ray spectroscopy confirmed the efficiency of the doping process by showing the ion-release kinetics. ICIE16-Cu exhibited a higher ion release than ICIE16-Sr. ICIE16-Cu induced low immune cell migration and triggered not only a low number of M1 and M2 macrophages but also of blood vessels. ICIE16-Sr induced higher numbers of M1 macrophages after 30 days. Both bioglass types induced numbers of M2 macrophages comparable with those found in the control groups.
CONCLUSION
Bioglass doping with copper and strontium did not significantly influence the foreign body response nor vascularization of the implantation bed in vivo. However, all the studied bioglass materials seemed to be biocompatible.
背景
生物玻璃是一种高度可接受的骨替代材料，可与所谓的治疗离子结合。然而，关于治疗离子对免疫反应和相关骨愈合的影响的知识还很缺乏。因此，本工作的目的是研究锶和铜掺杂生物玻璃对 M1 和 M2 巨噬细胞的诱导作用以及血管生成的影响。
材料与方法
采用熔融淬火法，在 ICIE16 生物玻璃中添加 5wt%的铜或锶，制备了两种类型的碱玻璃（ICIE16-Cu 和 ICIE16-Sr）。纯 ICIE16 和 45S5 生物玻璃作为对照材料。研究了生物玻璃的离子释放和化学成分，并在 Sprague-Dawley 大鼠的皮下进行了体内实验。
结果
扫描电子显微镜显示生物玻璃材料的表面形貌存在显著差异。能谱分析证实了掺杂过程的效率，显示了离子释放动力学。ICIE16-Cu 的离子释放率高于 ICIE16-Sr。ICIE16-Cu 表现出较低的免疫细胞迁移，不仅诱导了较少数量的 M1 和 M2 巨噬细胞，而且还诱导了较少数量的血管。ICIE16-Sr 在 30 天后诱导了更多的 M1 巨噬细胞。两种生物玻璃类型诱导的 M2 巨噬细胞数量与对照组相当。
结论
生物玻璃中铜和锶的掺杂并没有显著影响体内植入物床的异物反应和血管生成。然而，所有研究的生物玻璃材料似乎都是生物相容的。
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