Goodfellow Samuel M, Nofchissey Robert A, Morley Valerie J, Coan Kathryn E, Schwalm Kurt C, Cook Joseph A, Dunnum Jonathan L, Hanfelt-Goade Diane, Dinwiddie Darrell L, Domman Daryl B, Dragoo Jerry W, Kuhn Jens H, Bradfute Steven B
Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Museum of Southwestern Biology, Biology Department, University of New Mexico, Albuquerque, NM, USA.
Npj Viruses. 2024 Apr 4;2(1):11. doi: 10.1038/s44298-024-00016-6.
Orthohantaviruses infect distinct eulipotyphlan and rodent reservoirs throughout the world; some rodent orthohantaviruses can cause disease in humans. In the United States, a primary rodent reservoir for the human-pathogenic Sin Nombre virus (SNV) is the western deermouse (Peromyscus sonoriensis; formerly included in Peromyscus maniculatus). Deermice (rodents of genus Peromyscus) carry presumably distinct orthohantaviruses but, although deermice of ten species have been recorded in New Mexico, only SNV has been reported in rodents from that state. Using a set of pan-orthohantavirus primers, we discovered a non-SNV orthohantavirus in a brush deermouse (P. boylii), trapped in central New Mexico in 2019. Sequencing enabled the generation of a consensus coding-complete genome sequence, revealing similarity to the known partial sequences of the unclassified "Limestone Canyon virus (LSCV)" in GenBank and aligning with the information in an unpublished study of wild-caught brush deermice trapped in southwestern New Mexico in 2006. Phylogenetic analysis of these combined data revealed geospatial clades and overall identity of "LSCV", uncovering its association with the classified Montaño virus (MTNV), which is known to infect Aztec and Orizaba deermice in central Mexico. Our work emphasizes the importance of determining coding-complete viral genome sequences as a framework for rigorous virus classification as the basis for epidemiological studies.
正汉坦病毒在全球感染不同的真盲缺目动物和啮齿类宿主；一些啮齿类正汉坦病毒可导致人类患病。在美国，人类致病性辛诺柏病毒（SNV）的主要啮齿类宿主是西部鹿鼠（Peromyscus sonoriensis；以前包含在鹿鼠属Peromyscus maniculatus中）。鹿鼠（Peromyscus属啮齿动物）携带可能不同的正汉坦病毒，然而，尽管在新墨西哥州已记录到10种鹿鼠，但该州啮齿动物中仅报告了SNV。我们使用一组泛正汉坦病毒引物，于2019年在新墨西哥州中部捕获的一只灌丛林鹿鼠（P. boylii）中发现了一种非SNV正汉坦病毒。通过测序生成了一个一致的编码完整基因组序列，显示出与GenBank中未分类的“石灰岩峡谷病毒（LSCV）”已知部分序列相似，并与2006年在新墨西哥州西南部捕获的野生灌丛林鹿鼠的一项未发表研究中的信息相符。对这些合并数据的系统发育分析揭示了“LSCV”的地理空间进化枝和总体特征，发现它与已分类的蒙塔尼奥病毒（MTNV）相关，已知MTNV感染墨西哥中部的阿兹特克鹿鼠和奥里萨巴鹿鼠。我们的工作强调了确定编码完整的病毒基因组序列作为严格病毒分类框架的重要性，这是流行病学研究的基础。
