Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu 610065, China.
Int J Mol Sci. 2022 May 8;23(9):5241. doi: 10.3390/ijms23095241.
Drought decreases the inorganic phosphate (Pi) supply of soil, resulting in Pi starvation of plants, but the molecular mechanism of how plants, especially the perennial trees, are tolerant to drought stress and Pi starvation, is still elusive. In this study, we identified an AP2/ERF transcription factor gene, , from var. , and it was induced by both mannitol treatment and Pi starvation. Overexpressing and knocking-down of both enhanced and attenuated tolerance to drought stress and Pi deficiency compared to WT, respectively. Moreover, the overexpression of up-regulated the expression levels of Pi starvation-induced (PSI) genes and increased Pi uptake under drought conditions; however, its poplar showed the opposite phenotypes. Subsequent analysis indicated that PalERF2 directly modulated expressions of drought-responsive genes and , as well as PSI genes and , through binding to the DRE motifs on their promoters. These results clearly indicate that poplars can recruit to increase the tolerance to drought and also elevate Pi uptake under drought stress.
干旱会降低土壤中的无机磷酸盐（Pi）供应，导致植物出现 Pi 饥饿，但植物，尤其是多年生树木，如何耐受干旱胁迫和 Pi 饥饿的分子机制仍不清楚。在这项研究中，我们从 var. 中鉴定出一个 AP2/ERF 转录因子基因 ，它同时受到甘露醇处理和 Pi 饥饿的诱导。与 WT 相比，过表达和敲低 均增强和减弱了对干旱胁迫和 Pi 缺乏的耐受性。此外， 的过表达上调了 Pi 饥饿诱导（PSI）基因的表达水平，并在干旱条件下增加了 Pi 的摄取；然而，其 杨树表现出相反的表型。后续分析表明，PalERF2 通过与启动子上的 DRE 基序结合，直接调节干旱响应基因 和 以及 PSI 基因 和 的表达。这些结果清楚地表明，杨树可以招募 来提高对干旱的耐受性，并在干旱胁迫下提高 Pi 的摄取。
