Geda Abdo Megra, Wendimu Aregash, Lulie Solomon, Dessalegn Bereket, Tesfaw Liyuwork, Assefa Eyob, Birhanu Kenaw, Deresse Getaw, Dufera Dawit, Enbiyale Gashaw, Tesfaye Mulusew, Mihret Tadesse
Department of Veterinary Pathobiology, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, Ethiopia.
Department of Veterinary Clinical Medicine, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, Ethiopia.
Int J Microbiol. 2025 Apr 21;2025:8356389. doi: 10.1155/ijm/8356389. eCollection 2025.
Fowl cholera is a highly infectious bacterial disease in poultry production. It is caused by () and leads to significant health risks and financial losses. Therefore, this study is aimed at isolating, molecularly detecting, and analyzing the antibiogram of from breeder chickens in Gondar City. A cross-sectional study design with purposive sampling was employed to collect a total of 130 tracheal swab samples from breeder chickens showing clinical signs of fowl cholera between January 2023 and December 2023, based on case availability. Bacterial isolation was performed using bacteriological and biochemical tests. The isolated was confirmed through conventional polymerase chain reaction (PCR) using a capsular serotype-specific primer (). The antibiogram assessment of against 10 antimicrobial agents was conducted using the Kirby-Bauer disk diffusion method. Descriptive statistics were used to analyze the isolation rate of the bacterium. Of the 130 sampled swabs, 10 (7.69%) tested positive for in the phenotypic assay, and 3 (30%) of those isolates were positive for the virulence gene. The study found that all three isolates were 100% sensitive to penicillin, ampicillin, norfloxacin, and florfenicol, while showing 100% intermediate sensitivity to streptomycin and 66.7% intermediate sensitivity to gentamycin, amoxicillin, tetracycline, trimethoprim/sulphamethoxazole, and kanamycin. The study confirms that , the causative agent of fowl cholera in breeder chickens, is circulating in the area and exhibits varying antimicrobial sensitivity profiles.
禽霍乱是家禽生产中一种高度传染性的细菌性疾病。它由（）引起，会导致重大的健康风险和经济损失。因此，本研究旨在从贡德尔市的种鸡中分离、分子检测并分析（）的抗菌谱。采用目的抽样的横断面研究设计，根据病例可得性，于2023年1月至2023年12月期间从表现出禽霍乱临床症状的种鸡中总共采集了130份气管拭子样本。使用细菌学和生化试验进行细菌分离。使用荚膜血清型特异性引物（）通过常规聚合酶链反应（PCR）对分离出的（）进行确认。使用 Kirby - Bauer 纸片扩散法对（）针对10种抗菌剂的抗菌谱进行评估。使用描述性统计分析该细菌的分离率。在130份采样拭子中，有10份（7.69%）在表型检测中对（）呈阳性，其中3份（30%）分离株对（）毒力基因呈阳性。研究发现，所有三株分离株对青霉素、氨苄西林、诺氟沙星和氟苯尼考均100%敏感，而对链霉素100%呈中度敏感，对庆大霉素、阿莫西林、四环素、甲氧苄啶/磺胺甲恶唑和卡那霉素66.7%呈中度敏感。该研究证实，种鸡禽霍乱的病原体（）在该地区传播，并表现出不同的抗菌敏感性特征。
