HydroSciences Montpellier, Université de Montpellier, CNRS, IRD, Montpellier, France
Laboratoire d'Ecologie Microbienne Hospitalière, CHU Montpellier, Montpellier, France.
Appl Environ Microbiol. 2018 Nov 15;84(23). doi: 10.1128/AEM.01739-18. Print 2018 Dec 1.
spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, sources of these emergent CF pathogens remain unknown. A large collection of specimens ( = 273) was sampled in the homes of 3 CF patients chronically colonized by with the aim of evaluating the potential role of domestic reservoirs in sustaining airway colonization of the patients. Samples were screened for the presence of by using genus-specific molecular detection. Species identification, multilocus genotypes, and antimicrobial susceptibility patterns observed for environmental isolates were compared with those of clinical strains. Patient homes hosted a high diversity of species ( = 7), including and , two species previously isolated from human samples only, and genotypes ( = 15), all showing an overall susceptibility to antimicrobial agents. strains were mostly isolated from indoor moist environments and siphons, which are potential reservoirs for several CF emerging pathogens. , the worldwide prevalent species colonizing CF patients, was not the major species inhabiting domestic environments. genotypes chronically colonizing the patients were not detected in their household environments. These results support the notions that the domestic environment could not be incriminated in sustained patient colonization and that after initial colonization, the environmental survival of clones adapted to the CF airways is probably impaired. spp. are worldwide emerging opportunistic pathogens in CF patients, able to chronically colonize the respiratory tract. Apart from regular consultations at the hospital CF center, patients spend most of their time at home. Colonization from nonhuman sources has been suggested, but the presence of spp. in CF patients' homes has not been explored. The domestic environments of CF patients chronically colonized by , especially wet environments, host several opportunistic pathogens, including a large diversity of species and genotypes. However, genotypes colonizing the patients were not detected in their domestic environments, making it unlikely that a shuttle between environment and CF airways is involved in persisting colonization. This also suggests that once the bacteria have adapted to the respiratory tract, their survival in the domestic environment is presumably impaired. Nevertheless, measures for reducing domestic patient exposure should be targeted on evacuation drains, which are frequently contaminated by CF opportunistic pathogens.
种是不发酵革兰氏阴性杆菌，被认为是囊性纤维化（CF）中的新兴病原体。虽然已经描述了一些 CF 患者之间的交叉传播事件，但菌株主要是患者特异性的，表明是从非人类宿主中偶然获得的。然而，这些新兴 CF 病原体的来源仍然未知。从 3 名慢性定植于 CF 患者的患者家中采集了大量标本（=273），目的是评估家庭宿主在维持患者气道定植中的潜在作用。使用属特异性分子检测方法筛选标本中是否存在 。比较环境分离株的物种鉴定、多位点基因型和抗菌药物敏感性模式与临床分离株的结果。患者家中存在高度多样化的 种（=7），包括 和 ，这两种先前仅从人类样本中分离到，还有 15 种基因型，所有这些对抗菌药物均表现出总体敏感性。 菌株主要从室内潮湿环境和虹吸管中分离出来，这些都是几种 CF 新兴病原体的潜在宿主。 ，是定植 CF 患者的世界性流行种，并不是定植于家庭环境中的主要 种。慢性定植于患者的 基因型未在其家庭环境中检测到。这些结果支持以下观点：家庭环境不会导致患者持续定植，并且在初始定植后，适应 CF 气道的 克隆的环境存活能力可能受损。 种是 CF 患者中普遍存在的机会性病原体，能够长期定植于呼吸道。除了在医院 CF 中心定期就诊外，患者大部分时间都在家中度过。已经提出了从非人类来源的定植，但尚未探讨 CF 患者家中 是否存在 种。慢性定植于 的 CF 患者的家庭环境中存在多种机会性病原体，包括多种 和基因型。然而，未在患者的家庭环境中检测到定植于患者的 基因型，这表明环境和 CF 气道之间的穿梭定植不太可能发生。这也表明，一旦细菌适应了呼吸道，它们在家庭环境中的存活能力就会受到影响。然而，减少患者在家中接触的措施应针对引流渠，这些引流渠经常受到 CF 机会性病原体的污染。
