González-Llorente Lucía, Andrés-Gasco Miguel, Gil Aranda Macarena Alba, Rabadán-Ros Rubén, Zapata-Pérez Rubén, Núñez-Delicado Estrella, Menéndez-Coto Nerea, García-González Claudia, Baena-Huerta Francisco Javier, Coto-Montes Ana, Caso-Peláez Enrique
UCAM HiTech Sport & Health Innovation Hub, Universidad Católica de Murcia, Guadalupe de Maciascoque, 30107 Murcia, Spain.
System and Precision Medicine Unit, Hospital Ribera Covadonga, 33204 Gijón, Asturias, Spain.
Biomedicines. 2025 Apr 12;13(4):949. doi: 10.3390/biomedicines13040949.
: The exposome, encompassing all environmental influences on health, plays a pivotal role in oxidative stress-related diseases. Negative air ions (NAIs), generated via cold atmospheric plasma (CAP), have been proposed as potential modulators of oxidative resilience. This study aims to investigate the metabolic adaptations induced by prolonged exposure to an NAI-enriched environment in mice, focusing on its effects in oxidative stress markers and energy metabolism in liver and blood. : Twenty male C57BL/6J mice were divided into four groups: two experimental groups exposed to NAI-enriched air generated by an Air Cold Atmospheric Plasma-Nanoparticle Removal (aCAP-NR) device for either 18 days (short-term, ST) or 28 days (long-term, LT), and two control groups without exposure. Targeted metabolomics was performed in whole blood and liver using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). Statistical and pathway analyses were conducted to assess metabolic alterations. : Metabolic profiling revealed significant shifts in oxidative stress-related pathways, including enhanced glutathione metabolism, reduced lipid peroxidation, and modulation of purine metabolism. Short-term exposure led to increased mitochondrial efficiency and energy homeostasis, while long-term exposure induced adaptive metabolic reprogramming, with higher inosine levels suggesting enhanced antioxidant and anti-inflammatory responses. No adverse effects on systemic or hepatic health markers were observed. : NAI exposure via aCAP-NR elicits a hormetic response, enhancing metabolic efficiency and resilience to oxidative stress. These findings suggest that controlled environmental enrichment with NAIs may serve as a novel non-invasive strategy for mitigating oxidative damage and improving metabolic health, as hormetic adaptative capacity and resilience to oxidative stress, warranting further translational research.
暴露组涵盖了所有对健康的环境影响，在氧化应激相关疾病中起着关键作用。通过冷大气等离子体（CAP）产生的负空气离子（NAIs）已被提议作为氧化恢复力的潜在调节剂。本研究旨在调查小鼠长期暴露于富含NAI的环境中所诱导的代谢适应性，重点关注其对肝脏和血液中氧化应激标志物及能量代谢的影响。：将20只雄性C57BL/6J小鼠分为四组：两个实验组分别暴露于由空气冷大气等离子体 - 纳米颗粒去除（aCAP - NR）装置产生的富含NAI的空气中18天（短期，ST）或28天（长期，LT），另外两个对照组不进行暴露。使用超高效液相色谱 - 质谱联用（UHPLC - MS）对全血和肝脏进行靶向代谢组学分析。进行统计和通路分析以评估代谢改变。：代谢谱分析揭示了氧化应激相关通路的显著变化，包括谷胱甘肽代谢增强、脂质过氧化减少以及嘌呤代谢的调节。短期暴露导致线粒体效率和能量稳态增加，而长期暴露诱导适应性代谢重编程，肌苷水平升高表明抗氧化和抗炎反应增强。未观察到对全身或肝脏健康标志物的不良影响。：通过aCAP - NR暴露于NAI会引发一种应激反应，提高代谢效率和对氧化应激的恢复力。这些发现表明，通过控制环境中NAI的富集，可能作为一种新型的非侵入性策略来减轻氧化损伤并改善代谢健康，因为应激适应性能力和对氧化应激的恢复力值得进一步的转化研究。
