Menéndez-Coto Nerea, Garcia-Gonzalez Claudia, Baena-Huerta Francisco Javier, Zapata-Pérez Rubén, Rabadán-Ros Rubén, Núñez-Delicado Estrella, González-Llorente Lucía, Caso-Peláez Enrique, Coto-Montes Ana
Department of Morphology and Cell Biology, University of Oviedo, 33006 Oviedo, Spain.
Research Group Oxidative Stress Knowledge and Advanced Research (OSKAR), Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain.
Int J Mol Sci. 2024 Dec 3;25(23):12986. doi: 10.3390/ijms252312986.
Ageing leads to a gradual deterioration of the organs, with the brain being particularly susceptible, often leading to neurodegeneration. This process includes well-known changes such as tau hyperphosphorylation and beta-amyloid deposition, which are commonly associated with neurodegenerative diseases but are also present in ageing. These structures are triggered by earlier cellular changes such as energy depletion and impaired protein synthesis, both of which are essential for cell function. These changes may in part be induced by environmental pollution, which has been shown to accelerate these processes. Cold Atmospheric Plasma (CAP) or atmospheric pressure gas discharge plasmas have shown promise in activating the immune system and improving cellular function in vitro, although their effects at the organ level remain poorly understood. Our aim in this work is to investigate the effect of a device that combines CAP treatment with the effective removal of environmental nanoparticles, typical products of pollution, on the activity of aged mouse brains. The results showed an increase in energy capacity, a reduction in reticulum stress and an activation of cellular autophagic clearance, minimising aggresomes in the brain. This leads to a reduction in key markers of neurodegeneration such as tau hyperphosphorylation and beta-amyloid deposition, demonstrating the efficacy of the tested product at the brain level.
衰老会导致器官逐渐衰退，大脑尤其容易受到影响，常常引发神经退行性变。这个过程包括一些众所周知的变化，如tau蛋白过度磷酸化和β-淀粉样蛋白沉积，这些变化通常与神经退行性疾病有关，但也存在于衰老过程中。这些结构变化是由早期细胞变化引发的，如能量消耗和蛋白质合成受损，而这两者对细胞功能都至关重要。这些变化可能部分是由环境污染引起的，已有研究表明环境污染会加速这些过程。冷大气等离子体（CAP）或大气压气体放电等离子体在体外激活免疫系统和改善细胞功能方面已显示出前景，尽管它们在器官水平的作用仍知之甚少。我们这项工作的目的是研究一种将CAP处理与有效去除环境纳米颗粒（典型的污染产物）相结合的装置对老年小鼠大脑活动的影响。结果显示能量容量增加、内质网应激减轻以及细胞自噬清除激活，使大脑中的聚集体最小化。这导致神经退行性变的关键标志物如tau蛋白过度磷酸化和β-淀粉样蛋白沉积减少，证明了受试产品在大脑水平的功效。
