Kataoka T, Oh-hashi F, Tsukagoshi S, Sakurai Y
Cancer Res. 1977 Apr;37(4):964-8.
Immunization with L1210 mouse leukemia cells treated with glutaraldehyde and concanavalin A resulted in the induction of leukemia-specific immune resistance in BALB/c X DBA/2Cr F1 mice. This was measured by survival of the mice after challenge either with the intact cells or with modified leukemia cells of less malignancy. Factors involved in the induction of immunoprophylaxis were analyzed. To produce an immune response against L1210 cells, preincubation with both glutaraldehyde and concanavalin A was required. Repeated sensitization with this combination produced higher incidences of survivors upon subsequent challenge with L1210 cells than did a single sensitization by equivalent or larger doses. However, the sensitization was rendered less effective by repetition of the immunization process five times.
用戊二醛和伴刀豆球蛋白A处理的L1210小鼠白血病细胞进行免疫，可在BALB/c×DBA/2Cr F1小鼠中诱导出白血病特异性免疫抗性。这通过用完整细胞或恶性程度较低的改良白血病细胞攻击后小鼠的存活情况来衡量。对诱导免疫预防所涉及的因素进行了分析。为了产生针对L1210细胞的免疫反应，需要同时用戊二醛和伴刀豆球蛋白A进行预孵育。与同等剂量或更大剂量的单次致敏相比，用这种组合反复致敏在随后用L1210细胞攻击时产生的存活者发生率更高。然而，通过重复免疫过程五次，致敏效果会降低。
