GmERF071基因的过表达赋予大豆对大豆胞囊线虫的抗性。

Overexpression of the GmERF071 gene confers resistance to soybean cyst nematode in soybean.

作者信息

Xiong Erhui, Xu Jiaqi, Feng Pingzhang, Lian Yun, Zhi Xiaoling, Li Ke, Zhang Erhan, Li Bing, Zhao Shijie, Liu Changzhong, Wei Chengyu, Li Panpan, Zhao Yaping, Zhao Lipei, Zheng Mengwei, Zhang Heng, Li Yi, Chu Shanshan, Jiao Yongqing

机构信息

Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, China.

Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China.

出版信息

Plant Genome. 2025 Jun;18(2):e70033. doi: 10.1002/tpg2.70033.

DOI:10.1002/tpg2.70033

PMID:40302203

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12041739/

Abstract

Soybean cyst nematode (SCN) is one of the most harmful pests, causing major reductions in soybean yield globally. The validation and functional characterization of SCN resistance genes are crucial to improving soybean yield worldwide. Herein, we describe an SCN resistance gene, GmERF071 (Glyma.19g262700). GmERF071 is a hydrophilic, unstable protein with an AP2/ERF subfamily ethylene response transcription factor domain, which is localized in the nucleus. Overexpression of GmERF071 enhanced SCN resistance in the soybean stable genetic transformation and root systems. RNA-seq analysis revealed 394 upregulated and 132 downregulated differentially expressed genes (DEGs) in GmERF071 overexpression transgenic plants. The DEGs participated in plant-pathogen interactions, mitogen-activated protein kinase signaling, plant hormone signal transduction, response to chitin, response to carbohydrates, response to wounding in starch and sucrose metabolism, phenylpropionic acid biosynthesis, and flavonoid biosynthesis. Nine candidate DEGs were verified using real-time quantitative reverse transcription PCR. These results suggest that GmERF071 plays a key role in SCN resistance and could be used in genomics-assisted breeding to develop soybean varieties with increased resistance to SCN.

摘要

大豆胞囊线虫（SCN）是最具危害性的害虫之一，在全球范围内导致大豆产量大幅下降。SCN抗性基因的验证和功能表征对于提高全球大豆产量至关重要。在此，我们描述了一个SCN抗性基因GmERF071（Glyma.19g262700）。GmERF071是一种亲水性、不稳定的蛋白质，具有AP2/ERF亚家族乙烯响应转录因子结构域，定位于细胞核。GmERF071的过表达增强了大豆稳定遗传转化和根系对SCN的抗性。RNA测序分析揭示了GmERF071过表达转基因植物中有394个上调和132个下调的差异表达基因（DEG）。这些DEG参与植物-病原体相互作用、丝裂原活化蛋白激酶信号传导、植物激素信号转导、对几丁质的反应、对碳水化合物的反应、淀粉和蔗糖代谢中对伤口的反应、苯丙酸生物合成和类黄酮生物合成。使用实时定量逆转录PCR验证了9个候选DEG。这些结果表明，GmERF071在SCN抗性中起关键作用，可用于基因组辅助育种，以培育对SCN抗性增强的大豆品种。