Kalambhe Deepali Gopal, Kaur Harpreet, Gill Jatinder Paul Singh
Centre for One Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Directorate of Research, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Transbound Emerg Dis. 2024 Jan 23;2024:7550006. doi: 10.1155/2024/7550006. eCollection 2024.
In India, nematode worm remains highly neglected due to the paucity of research. Recent trichinellosis outbreaks in humans associated with pork meal in north India have highlighted an emerging food safety concern due to this neglected parasite.
This study aimed to ascertain the existence of species and to identify them in slaughtered pigs intended for human consumption in the Punjab and Uttaranchal regions of North India.
A total of 1,194 slaughter pig tongue samples were screened in 239 pools using the double separatory funnel method for recovery of larvae. The species of recovered larvae were confirmed by multiplex PCR assay and sequencing. A phylogenetic tree was constructed to ascertain the closest lineage.
This study reported 3.77% and 1.26% positivity in pooled and individual pig samples, respectively. Pigs slaughtered in Punjab reported higher positivity (1.73%) than pigs in Uttaranchal (0.81%). Among all places, Jalandhar recorded highest positivity of 5.66%, followed by 2.22% in Nainital and 0.8% in Patiala, whereas none of the pig samples from Chandigarh, Ludhiana, Bathinda, Tehri Garhwal, Pauri Garhwal, Kashipur, Haridwar was positive. Molecular confirmation by PCR and sequencing confirmed the recovered larvae as and parasite remained highly neglected in India; however, the detection of sylvatic species in pigs intended for human consumption indicated the emergence of zoonotic foodborne risk at wild animal-domestic pig and human interfaces. To the best of our knowledge, this is the first study reporting and . in pigs of North India. positivity in slaughtered pigs is an early alarm for the emergence of foodborne risk from this neglected parasitic worm. Considering the importance of pigs as a source of meat and their role as a reservoir for raised the food safety concern that warrants strict meat inspection and extensive studies on neglected parasites in India.
在印度，由于研究匮乏，线虫一直被严重忽视。印度北部近期发生的与猪肉粉相关的人类旋毛虫病疫情凸显了这种被忽视的寄生虫引发的新出现的食品安全问题。
本研究旨在确定印度北部旁遮普邦和北阿坎德邦供人类食用的屠宰猪中旋毛虫物种的存在并对其进行鉴定。
使用双分离漏斗法对239组共1194份屠宰猪舌样本进行筛查，以回收幼虫。通过多重聚合酶链反应（PCR）检测和测序确认回收幼虫的物种。构建系统发育树以确定最接近的谱系。
本研究报告在混合猪样本和单个猪样本中的阳性率分别为3.77%和1.26%。旁遮普邦屠宰的猪的阳性率（1.73%）高于北阿坎德邦的猪（0.81%）。在所有地点中，贾朗达尔的阳性率最高，为5.66%，其次是奈尼塔尔的2.22%和帕蒂亚拉的0.8%，而昌迪加尔、卢迪亚纳、巴辛达、特里加瓦尔、波里加瓦尔、卡西普尔、哈里德瓦尔的猪样本均未呈阳性。通过PCR和测序进行的分子确认证实回收的幼虫为旋毛虫属。旋毛虫属寄生虫在印度仍然被严重忽视；然而，在供人类食用的猪中检测到野生动物旋毛虫物种表明在野生动物 - 家猪和人类界面出现了人畜共患食源性风险。据我们所知，这是第一项在印度北部猪中报告旋毛虫属和某种旋毛虫的研究。屠宰猪中的阳性率是这种被忽视的寄生虫引发食源性风险出现的早期警报。考虑到猪作为肉类来源的重要性及其作为旋毛虫宿主的作用，引发了食品安全担忧，这需要严格的肉类检验以及对印度被忽视的寄生虫进行广泛研究。
