Igriczi Barbara, Dénes Lilla, Czétényi Anna, Révész Tamás, Somogyi Zoltán, Balka Gyula
Department of Pathology, University of Veterinary Medicine, István Str 2., Budapest, 1078, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, István Str 2., Budapest, 1078, Hungary.
Transbound Emerg Dis. 2024 Jan 3;2024:5534854. doi: 10.1155/2024/5534854. eCollection 2024.
In the last few decades, many new paramyxoviruses have been discovered, causing diverse, mostly respiratory diseases in animals and humans. The porcine parainfluenza virus 1 (PPIV-1, species ), which has been reported in many countries worldwide, was found in both healthy and clinically ill pigs showing respiratory signs. Here, we report the expected prevalence and genetic diversity of PPIV-1 in Hungarian pig herds and the detection in one Slovakian pig farm, which is the first report of evidence for the presence of the virus in the country. To estimate the prevalence in Hungary 211 oral fluid samples were collected from 23 large-scale swine herds in a systematic way and tested by real-time quantitative RT-PCR. The presence of the virus was detected in 10 of the 23 Hungarian farms (43%) included in our study. One hundred eighty-one nasal swab samples were collected cross-sectionally from three Hungarian and one Slovakian PPIV-1-positive herd and PPIV-1 was most prevalent in 6-week-old pigs on farms located in Hungary and in the 2-week-old pigs on the Slovakian farm. Phylogenetic analysis of three Hungarian and two Slovakian PPIV-1 F-gene sequences showed high-nucleotide identity (>93%) and all belonged to Clade I, together with the other European strains.
在过去几十年中，发现了许多新的副黏病毒，它们在动物和人类中引发了多种疾病，主要是呼吸道疾病。猪副流感病毒1型（PPIV-1，种）已在世界许多国家被报道，在出现呼吸道症状的健康猪和临床患病猪中均有发现。在此，我们报告了匈牙利猪群中PPIV-1的预期流行率和遗传多样性，以及在一个斯洛伐克猪场中的检测情况，这是该病毒在该国存在的首个证据报告。为了估计匈牙利的流行率，我们从23个大型猪群中系统收集了211份口腔液样本，并通过实时定量RT-PCR进行检测。在我们研究纳入的23个匈牙利农场中的10个（43%）检测到了该病毒的存在。从三个匈牙利和一个斯洛伐克的PPIV-1阳性猪群中横断面收集了181份鼻拭子样本，PPIV-1在匈牙利农场6周龄的猪中最为普遍，在斯洛伐克农场2周龄的猪中最为普遍。对三个匈牙利和两个斯洛伐克的PPIV-1 F基因序列进行系统发育分析，结果显示核苷酸同一性较高（>93%），并且与其他欧洲毒株一起均属于进化枝I。
