Department of Pathology, University of Veterinary Medicine, Budapest, Hungary.
CEVA-Phylaxia (Ceva Sante Animale), Budapest, Hungary.
Transbound Emerg Dis. 2021 Jul;68(4):1731-1735. doi: 10.1111/tbed.13869. Epub 2020 Oct 17.
Porcine respirovirus 1, also known as Porcine parainfluenza virus 1 (PPIV-1) was first identified in Hong Kong in 2013, later in the USA and most recently in Chile. Here, we report the first detection of PPIV-1 outside these three regions. We screened 22 farms in Hungary by testing 15 nasal swab samples obtained from 3-week-old piglets (3 randomly chosen piglets from 5 litters in each farm). Only one farm was found to be positive. We subsequently sampled the positive farm by taking cross-sectional 20 nasal swab samples from 2-, 4-, 6- and 8-week-old piglets. Virus detection by qRT-PCR showed that although all investigated age groups were positive to PPIV-1, a higher number of infected animals and higher viral loads were found among 4-week-old animals. Based on the phylogenetic analyses of partial F and L genes, the 3 Hungarian strains are genetically closely related to the very first PPIV-1 strain identified in Hong Kong in 2013, whereas the overall genetic difference compared to the recently described North American isolates was around 10%.
猪呼吸与繁殖综合征病毒 1 型，又称猪副流感病毒 1 型（PPIV-1）于 2013 年在香港首次被鉴定，随后在美国，最近在智利也有发现。在此，我们报告了该病毒在这三个地区以外的首次检出。我们通过检测来自 3 周龄仔猪（每个农场的 5 窝中随机选择的 3 只仔猪）的 15 个鼻拭子样本，对匈牙利的 22 个农场进行了筛查。仅发现一个农场呈阳性。随后，我们从 2、4、6 和 8 周龄的仔猪中采集了 20 个横向鼻拭子样本对该阳性农场进行了采样。qRT-PCR 检测显示，尽管所有调查的年龄组均对 PPIV-1 呈阳性，但在 4 周龄动物中发现了更多的感染动物和更高的病毒载量。基于 F 和 L 基因部分序列的系统进化分析，3 株匈牙利分离株与 2013 年在香港首次鉴定的首个 PPIV-1 株在遗传上密切相关，而与最近描述的北美分离株相比，整体遗传差异约为 10%。
