Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1850 Christensen Drive, Ames, IA 50011, USA.
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1850 Christensen Drive, Ames, IA 50011, USA; Department of Statistics, Iowa State University, 2438 Osborn Drive, Ames, IA 50011, USA.
Virology. 2021 Nov;563:88-97. doi: 10.1016/j.virol.2021.08.015. Epub 2021 Sep 3.
Two experimental challenge studies were conducted to evaluate the pathogenesis of a porcine parainfluenza virus type 1 (PPIV-1) isolate. Four-week-old conventional (CON) pigs were challenged in Study 1 and six-week-old caesarean derived/colostrum deprived (CDCD) pigs were challenged in Study 2. Results indicate that PPIV-1 shedding and replication occur in the upper and lower respiratory tracts of CON and CDCD pigs as detected by RT-qPCR and immunohistochemistry. Mild macroscopic lung lesions were observed in CON pigs but not in CDCD pigs. Microscopic lung lesions were mild and consisted of peribronchiolar lymphocytic cuffing and epithelial proliferation in CON and CDCD pigs. Serum neutralizing antibodies were detected in the CON and CDCD pigs by 14 and 7 days post inoculation, respectively. This study provides evidence that in spite of PPIV-1 infection and replication in challenged swine, significant clinical respiratory disease was not observed.
进行了两项实验性挑战研究，以评估猪副流感病毒 1 型（PPIV-1）分离株的发病机制。在研究 1 中，对 4 周龄常规（CON）猪进行了挑战，在研究 2 中，对剖腹产/初乳剥夺（CDCD）猪进行了挑战。结果表明，通过 RT-qPCR 和免疫组织化学检测，PPIV-1 在 CON 和 CDCD 猪的上呼吸道和下呼吸道中脱落和复制。在 CON 猪中观察到轻微的宏观肺部病变，但在 CDCD 猪中未观察到。CON 和 CDCD 猪的肺部病变轻微，包括细支气管周围淋巴细胞袖套和上皮增生。在 CON 和 CDCD 猪中，分别在接种后 14 天和 7 天检测到血清中和抗体。这项研究提供了证据，表明尽管在受挑战的猪中感染和复制了 PPIV-1，但未观察到明显的临床呼吸道疾病。
