Li Jinhao, Xu Hu, Li Chao, Zhao Jing, Gong Bangjun, Sun Qi, Guo Zhenyang, Zhang Siyu, Zhang Menglin, Xiang Lirun, Tang Yan-Dong, Wu Jianan, Wang Qian, Peng Jinmei, Zhou Guohui, Liu Huairan, An Tongqing, Cai Xuehui, Tian Zhi-Jun, Zhang Hongliang
State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001China.
Transbound Emerg Dis. 2024 May 31;2024:2969771. doi: 10.1155/2024/2969771. eCollection 2024.
Porcine reproductive and respiratory syndrome virus (PRRSV) critically threatens the pig industry in China. Lineage 1 PRRSV, which is divided into L1A-L1F and L1H-L1J, is widely recognized as the most extensively genetically diverse lineage globally. L1A (NADC34-like) and L1C (NADC30-like) PRRSVs have become the current major circulating strains in China. Notably, strains from other branches of L1 have not been reported in China. During our epidemiological investigation of PRRSV, we identified a new strain named HLJDZD55. Phylogenetic analysis of the ORF5 gene revealed that HLJDZD55 belongs to the L1B branch. Alignment of deduced amino acid sequences based on the Nsp2 gene indicated that HLJDZD55 has a discontinuous deletion of 131 amino acids (111 + 1 + 19). We further sequenced the whole genome of HLJDZD55, and phylogenetic analysis based on the whole-genome sequence revealed that HLJDZD55 belongs to the L1C branch. Recombination analysis of the whole genome demonstrated that HLJDZD55 is a recombinant strain of TJZH-1607 (L1C, identified in China) and Minnesota 14 (L1B, identified in the USA). These findings suggested that HLJDZD55 is a newly emerged lineage 1 PRRSV in China and is closely related to L1B PRRSV in the US, which may have been introduced from the U.S. strain and subsequently recombined with the local Chinese strain and underwent evolution. Taken together, these results demonstrated the emergence of L1B PRRSV in China for the first time.
猪繁殖与呼吸综合征病毒（PRRSV）对中国养猪业构成严重威胁。1型PRRSV分为L1A - L1F和L1H - L1J，被广泛认为是全球遗传多样性最广泛的毒株。L1A（NADC34样）和L1C（NADC30样）PRRSV已成为中国目前主要的流行毒株。值得注意的是，在中国尚未报道过L1其他分支的毒株。在我们对PRRSV的流行病学调查中，我们鉴定出一株名为HLJDZD55的新毒株。对ORF5基因的系统发育分析表明，HLJDZD55属于L1B分支。基于Nsp2基因推导的氨基酸序列比对表明，HLJDZD55有131个氨基酸的不连续缺失（111 + 1 + 19）。我们进一步对HLJDZD55的全基因组进行测序，基于全基因组序列的系统发育分析表明，HLJDZD55属于L1C分支。全基因组的重组分析表明，HLJDZD55是TJZH - 1607（在中国鉴定的L1C）和明尼苏达14（在美国鉴定的L1B）的重组毒株。这些发现表明，HLJDZD55是中国新出现的1型PRRSV，与美国的L1B PRRSV密切相关，可能是从美国毒株引入，随后与中国本土毒株重组并进化。综上所述，这些结果首次证明了L1B PRRSV在中国的出现。
