Han Yu, Adam Fathalrhman Eisa Addoma, Zhang Riteng, Gao Yan, Lei Jianlin, Lu Kejia, Wang Xinglong, Xiao Sa, Liu Haijin, Yang Zengqi
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Yulin Animal Husbandry and Veterinary Service Center, Yulin, China.
Transbound Emerg Dis. 2023 Nov 23;2023:8854528. doi: 10.1155/2023/8854528. eCollection 2023.
Bovine parainfluenza virus type 3 (BPIV3) is a significant pathogen in cattle, causing a range of respiratory symptoms from mild to severe. In this study, we isolated a BPIV3 (BPIV3/SX/2021) strain using Madin-Darby bovine kidney cells from a lung tissue sample obtained in Shaanxi province. The full length of the BPIV3/SX/2021 strain is 15,474 nt, encoding six structural and three accessory proteins. A phylogenetic analysis revealed that SX2021strain belongs to BPIV3 genotype C, and the isolate shares the highest sequence homology with the other genotype C strains isolated in China. A genetic comparison of the BPIV3c strain isolated in China showed that some amino acid residues have mutations in the F and HN genes that affect the protein structure. Animal challenge experiments showed that experimental infection of guinea pigs with the BPIV3c strain SX2021 displayed depression and dyspnea clinical signs. Histopathological analysis of the lung and trachea showed that morphological changes consisted of lesions of bronchial epithelial cell shedding, alveoli septa thickening, focal fiber hyperplasia, and macrophage infiltration. The virus load analysis indicated that the SX2021 strain displayed a higher replication level in the lungs and trachea. These results provide strong evidence that the evolution of BPIV3, and the isolated BPIV3 strain SX2021 provide useful data to determine the pathogenesis of the virus.
牛副流感病毒3型（BPIV3）是牛群中的一种重要病原体，可引发一系列从轻度到重度的呼吸道症状。在本研究中，我们使用来自陕西省的一份肺组织样本，通过马-达二氏牛肾细胞分离出一株BPIV3（BPIV3/SX/2021）毒株。BPIV3/SX/2021毒株全长15474 nt，编码六种结构蛋白和三种辅助蛋白。系统发育分析显示，SX2021毒株属于BPIV3基因型C，该分离株与在中国分离出的其他基因型C毒株具有最高的序列同源性。对中国分离的BPIV3c毒株进行基因比较发现，F和HN基因中的一些氨基酸残基发生了突变，影响了蛋白质结构。动物攻毒实验表明，用BPIV3c毒株SX2021对豚鼠进行实验性感染，豚鼠表现出抑郁和呼吸困难的临床症状。对肺和气管的组织病理学分析表明，形态学变化包括支气管上皮细胞脱落、肺泡间隔增厚、局灶性纤维增生和巨噬细胞浸润。病毒载量分析表明，SX2021毒株在肺和气管中表现出较高的复制水平。这些结果为BPIV3的进化提供了有力证据，分离出的BPIV3毒株SX2021为确定该病毒的发病机制提供了有用的数据。
