从中国牛中分离鉴定出牛副流感病毒 3 型。
Isolation and genetic characterization of bovine parainfluenza virus type 3 from cattle in China.
机构信息
Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China.
出版信息
Vet Microbiol. 2011 May 5;149(3-4):446-51. doi: 10.1016/j.vetmic.2010.11.011. Epub 2010 Nov 13.
Bovine parainfluenza virus type 3 (BPIV3) is one of the most important of the known viral respiratory pathogens of both young and adult cattle. However BPIV3 has not been detected or isolated in China prior to this study. In 2008, four BPIV3 strains were isolated with MDBK cells from cattle in China and characterized by RT-PCR, nucleotide sequence analysis, transmission electron microscope observation, hemadsorption and hemagglutination tests. Nucleotide phylogenetic analysis of partial hemagglutinin-neuraminidase (HN) gene for four isolates and the complete genome for the SD0835 isolate implicated that the four Chinese BPIV3 strains were distinct from the previously reported genotype A (BPIV3a) and genotype B (BPIV3b) and might be a potentially new genotype, which was tentatively classified as genotype C (BPIV3c). This is the first study to report the isolation and genetic characterization of BPIV3 from cattle in China.
牛副流感病毒 3 型（BPIV3）是已知的重要牛呼吸道病毒病原体之一，可感染幼龄牛和成年牛。然而，在本研究之前，中国尚未检测到或分离到 BPIV3。2008 年，我们从中国的牛中用 MDBK 细胞分离到了 4 株 BPIV3，并通过 RT-PCR、核苷酸序列分析、透射电镜观察、血凝和血凝抑制试验进行了鉴定。对 4 株分离株的部分血凝素-神经氨酸酶（HN）基因和 SD0835 分离株的全基因组进行核苷酸系统进化分析表明，这 4 株中国 BPIV3 与先前报道的基因型 A（BPIV3a）和基因型 B（BPIV3b）明显不同，可能是一种潜在的新基因型，暂定为基因型 C（BPIV3c）。这是首次在中国牛中分离和遗传特征分析 BPIV3 的研究。
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