Wen Yong-Jun, Shi Xin-Chuan, Wang Feng-Xue, Wang Wei, Zhang Shu-Qin, Li Guo, Song Ni, Chen Li-Zhi, Cheng Shi-Peng, Wu Hua
State Key Laboratory for Molecular Biology of Special Economic Animals, Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences (CAAS), 4899 Juye Street, Changchun, Jilin, China.
Virus Genes. 2012 Dec;45(3):542-7. doi: 10.1007/s11262-012-0810-1. Epub 2012 Aug 26.
In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains, the NM09 virus consisted of six non-overlapping genes, which were predicted to encode nine proteins with conserved and complementary 3' leader and 5' trailer regions, conserved gene starts, gene stops, and trinucleotide intergenic sequences. Nucleotide phylogenetic analysis of matrix and hemagglutinin-neuraminidase gene demonstrated that this NM09 isolate belonged to BPIV3 genotype A instead of the previously reported BPIV3 genotype C in China. It is implicated that the different genotypes A and C might coexist infection for a long time in China.
2009年，从中国正常牛的鼻拭子中，利用MDBK细胞培养分离出一株牛副流感病毒3型（BPIV3），命名为NM09。通过逆转录聚合酶链反应（RT-PCR）和核苷酸序列分析对NM09分离株进行了鉴定。其完整基因组长度为15456个核苷酸。与其他已测序的副流感病毒株相似，NM09病毒由六个不重叠的基因组成，预计这些基因编码九种蛋白质，具有保守且互补的3'前导区和5'尾随区、保守的基因起始、基因终止和三核苷酸基因间序列。基质蛋白和血凝素神经氨酸酶基因的核苷酸系统发育分析表明，该NM09分离株属于BPIV3 A基因型，而非中国此前报道的BPIV3 C基因型。这表明不同的A和C基因型可能在中国长期共存感染。
