Yang Taotao, Lin Anqi, Cui Shuoyan, Chen Jing, Wan Hanhao, Lu Yingmei, Li Pengcheng, Zhang Zhibang, Li Kai, Guo Minhong, Xu Yanzhang
College of Life Sciences and Resource Environment, Yichun University, Yichun 336000, Jiangxi, China.
Jiangxi Sunshine Dairy Company Limited, Nanchang 330043, Jiangxi, China.
Transbound Emerg Dis. 2023 Dec 11;2023:9977581. doi: 10.1155/2023/9977581. eCollection 2023.
Porcine teschovirus (PTV) is a member of the genus in the family . Several novel PTVs in were identified in our previous study. To elucidate the taxonomic status of the species and subtypes, PTV-positive samples were inoculated into porcine kidney (PK-15) and swine testicular (ST) cells. A total of 58 PTV strains were successfully isolated and 21 different VP1 gene sequences were obtained. A phylogenetic analysis of the VP1 gene revealed that these PTV isolates comprised 11 genotypes, including 10 known genotypes (PTV 1-6, PTV 8-9, PTV 11, and Teschovirus B3), and a newly identified genotype. A nearly complete genome sequence of the novel PTV genotype isolate, PTV-SG2, was obtained. The homologies of the nucleotides and amino acids between PTV-SG2 and other PTVs were 69.7%-85.0% and 76.1%-90.4%, respectively. A phylogenetic analysis of the P1, polyprotein, and 3 CD genes revealed that PTV-SG2 belonged to a novel genotype within , and a genetic divergence analysis of the novel PTV isolate further verified its taxonomic status. Based on the PTV naming convention, the novel PTV genotype identified in the present study was provisionally named PTV 19. Serum neutralization tests indicated that PTV 19 is a serotype-specific and completely different from previously reported PTV serotypes. Therefore, PTV 19 is a novel PTV serogroup. In conclusion, this study identified a PTV genotype and serotype within .
猪捷申病毒（PTV）是 科 属的一个成员。我们之前的研究中鉴定出了几种新型的猪捷申病毒。为了阐明该病毒种和亚型的分类地位，将PTV阳性样本接种到猪肾（PK - 15）细胞和猪睾丸（ST）细胞中。共成功分离出58株PTV毒株，并获得了21个不同的VP1基因序列。VP1基因的系统发育分析表明，这些PTV分离株包括11个基因型，其中包括10个已知基因型（PTV 1 - 6、PTV 8 - 9、PTV 11和捷申病毒B3）以及一个新鉴定的基因型。获得了新型PTV基因型分离株PTV - SG2的近乎完整的基因组序列。PTV - SG2与其他PTV之间核苷酸和氨基酸的同源性分别为69.7% - 85.0%和76.1% - 90.4%。P1、多聚蛋白和3个CD基因的系统发育分析表明，PTV - SG2属于 内的一个新型基因型，新型PTV分离株的遗传分化分析进一步验证了其分类地位。根据PTV命名惯例，本研究中鉴定的新型PTV基因型暂命名为PTV 19。血清中和试验表明，PTV 19具有血清型特异性，与先前报道的PTV血清型完全不同。因此，PTV 19是一个新型的PTV血清群。总之，本研究在 内鉴定出了一种PTV基因型和血清型。
