Godal H C, Nyvold N, Rustad A
Scand J Haematol. 1979 Jul;23(1):55-8. doi: 10.1111/j.1600-0609.1979.tb02854.x.
The osmotic fragility of red blood cells is influenced by even modest environmental changes. Consequently, the technical procedure must be strictly standardized. This implies that temperature equilibrium of the buffered salt solutions should be reached prior to the addition of blood. Furthermore, since erroneous statements concerning the composition of phosphate buffers regularly used to secure correct pH of the salt solutions have repeatedly appeared in the literature, pH control of such solutions prior to use becomes essential.
即使是适度的环境变化也会影响红细胞的渗透脆性。因此，技术操作程序必须严格标准化。这意味着在加入血液之前，缓冲盐溶液应达到温度平衡。此外，由于文献中经常出现关于用于确保盐溶液正确pH值的磷酸盐缓冲液组成的错误表述，因此在使用前对这类溶液进行pH值控制就变得至关重要。
