Sha Hanyu, Duan Qun, Lyu Dongyue, Qian Fang, Zheng Xiaojin, Guo Jiazhen, He Zhaokai, Lu Xinmin, Bukai Asaiti, Qin Shuai, Duan Ran, Guli Saier, Zhang Peng, Xiao Meng, Jing Huaiqi, Wang Xin
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Department of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Microbiol Spectr. 2025 Jun 3;13(6):e0286224. doi: 10.1128/spectrum.02862-24. Epub 2025 Apr 30.
Brucellosis, caused by the intracellular bacterium , often leads to chronic infection with recurrent symptoms, posing significant therapeutic challenges. Effective monitoring of antibody dynamics is crucial for understanding disease progression and enhancing clinical outcomes. To investigate the longitudinal dynamics of antibody titers and antigen-specific responses in animal husbandry practitioners exposed to brucellosis in Gansu, China. We conducted a serological study involving 400 animal husbandry workers, identifying 21 seropositive individuals (5.25%). Antibody titers and antigen-specific responses to lipopolysaccharide (LPS) and BP26 protein were assessed using the Rose-Bengal test (RBT), serum agglutination test (SAT), and western blotting. Acute brucellosis patients displayed a distinct three-phase antibody titer pattern: a rapid rise (12-38 days), a rapid decline (19-41 days), and a slow decline (42-148 days). BP26-specific antibodies persisted significantly longer than LPS antibodies, remaining detectable up to 395 days post-infection. Early treatment reduced acute-phase antibody titers, correlating with disease control. This study combined dynamic antibody titer monitoring with specific antigen monitoring, enhancing the accuracy and specificity of brucellosis diagnosis and offering a basis for chronic infection detection. The findings are highly significant for clinical practice and public health strategies, particularly in high-incidence areas, prevention and control, as well as high-risk population surveillance.
, a zoonotic intracellular bacterium, poses significant threats to both human health and economic security. Clinically, brucellosis can be challenging to differentiate from other long-term febrile illnesses, necessitating prompt and standardized treatment to prevent chronic persistent infections and multi-organ damage, which are notoriously difficult to treat. Thus, gaining a comprehensive understanding of the disease's progression is essential for early diagnosis and effective treatment strategies. This paper delves into the dynamics of serum antibody titers in patients with acute brucellosis, shedding light on the temporal patterns of antibody titers. Such insights are pivotal for monitoring disease progression and assessing the efficacy of treatment interventions. Furthermore, through western blotting analysis, the study reveals that antibodies against the BP26 protein in brucellosis patients persist over an extended period, which is helpful to identify the durable immune response of brucellosis and provides a theoretical basis for vaccine development and treatment strategy formulation.
布鲁氏菌病由细胞内细菌引起，常导致慢性感染并伴有反复症状，带来重大治疗挑战。有效监测抗体动态对于了解疾病进展和改善临床结果至关重要。为了研究中国甘肃接触布鲁氏菌病的畜牧业从业者抗体滴度和抗原特异性反应的纵向动态。我们对400名畜牧业工人进行了一项血清学研究，确定了21名血清阳性个体（5.25%）。使用虎红试验（RBT）、血清凝集试验（SAT）和western印迹法评估抗体滴度以及对脂多糖（LPS）和BP26蛋白的抗原特异性反应。急性布鲁氏菌病患者呈现出独特的三相抗体滴度模式：快速上升期（12 - 38天）、快速下降期（19 - 41天）和缓慢下降期（42 - 148天）。BP26特异性抗体持续时间明显长于LPS抗体，在感染后长达395天仍可检测到。早期治疗降低了急性期抗体滴度，与疾病控制相关。本研究将动态抗体滴度监测与特异性抗原监测相结合，提高了布鲁氏菌病诊断的准确性和特异性，并为慢性感染检测提供了依据。这些发现对临床实践和公共卫生策略具有高度重要性，特别是在高发病地区的预防控制以及高危人群监测方面。
布鲁氏菌作为一种人畜共患的细胞内细菌，对人类健康和经济安全构成重大威胁。临床上，布鲁氏菌病可能难以与其他长期发热疾病区分开来，需要及时进行标准化治疗以预防慢性持续性感染和多器官损伤，而这些情况 notoriously difficult to treat（此处英文有误，推测应为“ notoriously difficult to treat”，意为“ notoriously difficult to treat”，即“极难治疗”）。因此，全面了解疾病进展对于早期诊断和有效的治疗策略至关重要。本文深入研究了急性布鲁氏菌病患者血清抗体滴度的动态变化，揭示了抗体滴度的时间模式。这些见解对于监测疾病进展和评估治疗干预效果至关重要。此外，通过western印迹分析，该研究表明布鲁氏菌病患者针对BP26蛋白的抗体持续时间较长，这有助于识别布鲁氏菌病的持久免疫反应，并为疫苗开发和治疗策略制定提供理论依据。
