评估APTIMA Combo 2™试剂盒用于检测冷冻精液中的沙眼衣原体和淋病奈瑟菌。
Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen.
作者信息
Lutz Thibaut, Chautard Robin, Lafontaine Maxime, Genc Merve, Touati Arabella, Pereyre Sabine, Peuchant Olivia, Bébéar Cécile, Garnier Fabien, Hantz Sébastien
机构信息
Reproductive Biology Department, CHU Limoges, Limoges, France.
Bacteriology Department, National Reference Center for Bacterial STIs, CHU Bordeaux, Bordeaux, France.
出版信息
Eur J Clin Microbiol Infect Dis. 2025 Apr 30. doi: 10.1007/s10096-025-05138-0.
Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.
检测冷冻保存精液中的沙眼衣原体（CT）和淋病奈瑟菌（NG）对于精子捐献者的筛查至关重要。对添加了浓度分别为1至106 IFU/mL或CFU/mL的CT和NG的冷冻保存精液样本进行了APTIMA Combo 2™试剂盒（Panther，Hologic）检测限（LOD）的评估。在单一感染或混合感染中，CT的LOD为102 IFU/mL，NG的LOD为10 CFU/mL。研究突出了精液对扩增的抑制作用。本研究证实了APTIMA Combo 2™试剂盒在捐献前筛查冷冻保存精子样本方面的性能。
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