Tang Junyu, Liang Qizhao, Zhang Rui, Huang Xiaoping
College of Food Science, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China.
J Appl Genet. 2025 Apr 30. doi: 10.1007/s13353-025-00969-1.
The preservation of marine specimens requires effective methods to ensure research accuracy and ecological sustainability. However, conventional preservatives (e.g., formaldehyde) pose environmental and health risks due to their toxicity. In this study, we isolated Aspergillus amstelodami from the surface of Oreochromis mossambicus specimens and evaluated the antifungal potential of 1,2-benzoisothiazolin-3-one and Kathon. Both agents exhibited strong inhibitory effects on fungal growth, as evidenced by clear inhibition zones. Transcriptomic analysis revealed: (1) upregulation of detoxification-related genes, including cytochrome P450-mediated xenobiotic/drug metabolism, ABC transporters, and two-component systems, and (2) downregulation of ribosome biogenesis genes, impairing protein synthesis in Aspergillus amstelodami. In conclusion, this study provides novel insights into the molecular antifungal mechanisms of isothiazolone antimicrobial agents in combating contamination of marine biological specimens caused by Aspergillus amstelodami.
海洋标本的保存需要有效的方法来确保研究准确性和生态可持续性。然而，传统防腐剂（如甲醛）因其毒性会带来环境和健康风险。在本研究中，我们从莫桑比克罗非鱼标本表面分离出阿姆斯特丹曲霉，并评估了1,2-苯并异噻唑啉-3-酮和凯松的抗真菌潜力。两种试剂均对真菌生长表现出强烈的抑制作用，抑菌圈清晰可见。转录组分析显示：（1）解毒相关基因上调，包括细胞色素P450介导的异生物质/药物代谢、ABC转运蛋白和双组分系统；（2）核糖体生物发生基因下调，损害了阿姆斯特丹曲霉的蛋白质合成。总之，本研究为异噻唑啉抗菌剂对抗由阿姆斯特丹曲霉引起的海洋生物标本污染的分子抗真菌机制提供了新见解。
