Deng Zhaomin, Pu Lu, Ma Yongxin, Jiang Hao
Laboratory for Aging and Cancer Research, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China.
Laboratory for Aging and Cancer Research, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
PLoS One. 2025 Apr 30;20(4):e0321875. doi: 10.1371/journal.pone.0321875. eCollection 2025.
Liver cancer stands as one of the most pervasive and aggressive malignant neoplasms on a global scale, with hepatocellular carcinoma (HCC) accounting for over 90% of primary liver cancer cases. It has been documented that long non-coding RNA (lncRNA) plays a significant role in various pathological contexts and serves as a pivotal determinant in numerous malignancies, including HCC. Leveraging mRNA sequencing data and lncRNA expression profiles from 346 HCC samples and 50 pairs of adjacent normal samples sourced from the TCGA database, a comprehensive bioinformatics analysis was conducted. The dataset was randomly partitioned into a training subset and a validation subset. This rigorous analysis aimed to pinpoint the lncRNAs intricately linked to the oncogenesis of HCC, unveiling distinct expression patterns of these lncRNAs between normal liver and HCC samples across different stages. Moreover, the construction of a lncRNA-mRNA co-expression network led to the identification of five central lncRNA hubs (AC091057, AC099850, AC012073, DDX11-AS1, and AL035461). Subsequently, these five lncRNAs were validated using the independent validation set. In summary, this investigation successfully discerned five lncRNAs closely associated with the oncogenesis of HCC, thereby shedding light on their potential utility as diagnostic or therapeutic targets for this formidable disease.
肝癌是全球范围内最普遍且侵袭性最强的恶性肿瘤之一，其中肝细胞癌（HCC）占原发性肝癌病例的90%以上。有文献记载，长链非编码RNA（lncRNA）在各种病理情况下发挥着重要作用，并且在包括HCC在内的众多恶性肿瘤中是一个关键决定因素。利用来自TCGA数据库的346例HCC样本和50对相邻正常样本的mRNA测序数据和lncRNA表达谱，进行了全面的生物信息学分析。该数据集被随机分为训练子集和验证子集。这项严谨的分析旨在找出与HCC肿瘤发生密切相关的lncRNA，揭示这些lncRNA在正常肝脏和不同阶段的HCC样本之间的不同表达模式。此外，通过构建lncRNA-mRNA共表达网络，确定了五个核心lncRNA枢纽（AC091057、AC099850、AC012073、DDX11-AS1和AL035461）。随后，使用独立验证集对这五个lncRNA进行了验证。总之，本研究成功识别出五个与HCC肿瘤发生密切相关的lncRNA，从而为它们作为这种严重疾病的诊断或治疗靶点的潜在用途提供了线索。
