Zhao Qianyun, Liu Yubin, Li Mingxing, Zhao Long, Wang Tiangang, Xiao Yaqin, Wei Shulin, Wu Ke, Yang Jiayue, Wang Yaqiu, Zhao Yueshui, Du Fukuan, Chen Yu, Deng Shuai, Shen Jing, Xiao Zhangang, Li Zhi, Wang Shengpeng, Wu Xu
Cell Therapy & Cell Drugs of Luzhou Key Laboratory, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China; Department of Pharmacy, Maternal and Child Health Care And Family Planning Service Center, 620000 Meishan, Sichuan, China.
Cell Therapy & Cell Drugs of Luzhou Key Laboratory, Department of Pharmacology, School of Pharmacy, Southwest Medical University, 646000 Luzhou, Sichuan, China.
Carbohydr Polym. 2025 Jul 1;359:123570. doi: 10.1016/j.carbpol.2025.123570. Epub 2025 Apr 4.
Hawthorn fruit is rich in pectins; however, the gut microbiota-modulating effects of hawthorn pectin remain unclear. In this study, enzyme-extracted hawthorn pectin (M: 126.24 kDa; HG/RG-I ratio: 55.64 %/37.93 %; branching degree: 2.44) was fractionated into two distinct components: D1 (spherical, 345.76 kDa, HG/RG-I 57.48 %/37.25 %, branching degree 2.16) and D2 (flexible, 17.20 kDa, HG/RG-I 78.24 %/21.76 %, branching degree 1.33). Hawthorn pectin directly stimulated Dubosiella newyorkensis (D. newyorkensis) and Bifidobacterium bifidum (B. bifidum) growth in vitro. In hyperlipidemic mice, it alleviated dyslipidemia and enriched the abundance of Lactobacillus and Dubosiella. Parallel experiments confirmed that D. newyorkensis supplementation similarly alleviated dyslipidemia and increased Lactobacillus abundance, which was mediated by bacterial acetate production. Notably, only D1 emulated the pectin's prebiotic activity, promoting D. newyorkensis and B. bifidum growth. The high-RG-I D1, with elevated branching and larger M, was identified as the key driver of hawthorn pectin's prebiotic effect. These findings highlight that hawthorn pectin, particularly D1, specifically targets probiotic D. newyorkensis to synergize with Lactobacillus against hyperlipidemia.
山楂果实富含果胶；然而，山楂果胶对肠道微生物群的调节作用仍不清楚。在本研究中，酶提取的山楂果胶（分子量：126.24 kDa；HG/RG-I比例：55.64%/37.93%；分支度：2.44）被分离成两个不同的组分：D1（球形，345.76 kDa，HG/RG-I 57.48%/37.25%，分支度2.16）和D2（柔性，17.20 kDa，HG/RG-I 78.24%/21.76%，分支度1.33）。山楂果胶在体外直接刺激纽约杜氏菌（D. newyorkensis）和两歧双歧杆菌（B. bifidum）的生长。在高脂血症小鼠中，它减轻了血脂异常并增加了乳酸杆菌和杜氏菌的丰度。平行实验证实，补充D. newyorkensis同样减轻了血脂异常并增加了乳酸杆菌的丰度，这是由细菌产生乙酸介导的。值得注意的是，只有D1模拟了果胶的益生元活性，促进了D. newyorkensis和B. bifidum的生长。具有较高分支度和较大分子量的高RG-I D1被确定为山楂果胶益生元作用的关键驱动因素。这些发现突出表明，山楂果胶，特别是D1，特异性靶向益生菌D. newyorkensis，与乳酸杆菌协同对抗高脂血症。
