Alsaedi Sarah, Skogstad Marit, Haugen Fred
Division of Work Psychology and Physiology, National Institute of Occupational Health (STAMI), Oslo, Norway.
National Institute of Occupational Health (STAMI), Oslo, Norway.
BMJ Open. 2025 Apr 29;15(4):e098896. doi: 10.1136/bmjopen-2025-098896.
Evidence links night shift work to circadian rhythm disruption, causing hormonal and metabolic alterations, as well as increased risk for cardiovascular disease (CVD). This study investigates whether night shift work affects blood glucose variability and dysregulation, potentially driven by circadian misalignment. It also examines whether such disruptions elevate inflammatory markers involved in atherosclerosis and contribute to the exacerbation of CVD risk markers.
The study includes 60 participants: rotating night shift workers (day, evening, and night shifts) and day workers (controls) at a pharmaceutical plant. We will assess the effects of night shift work on metabolic and cardiovascular health over three phases: an initial 6-week observational period (phase I), baseline registration of CVD risk factors (phase II), and follow-up assessment of CVD risk factors at 2 years (phase III). Phase I registrations include working hours derived from payroll data, sleep metrics by OURA ring (actigraphy, plethysmography and temperature), continuous assessments of blood glucose using continuous glucose monitor, self-reported food diary and measurements of circadian rhythm markers (monocyte mRNA expression). In phases II and III, blood CVD risk factors such as markers of inflammation, lipids, glycosylated haemoglobin, D-dimer, clinical examination of blood pressure, resting heart rate, arterial stiffness by the means of carotid to femoral pulse wave velocity, carotid intima-media thickness and maximal oxygen uptake (V̇O) are measured. To this end, a comprehensive set of methods will be used in a prospective manner to provide new knowledge on shift work-induced glucose regulation and CVD risk factors.
All participants provided written informed consent prior to participating in the study, which will adhere to the principles outlined in the Declaration of Helsinki. Ethical approval has been granted by the Norwegian Regional Committee for Medical Research Ethics South-East B (reference # 745702). Dissemination plans include academic and public publications, as well as collaborations with national and regional policy-makers.
有证据表明，夜班工作与昼夜节律紊乱有关，会导致激素和代谢改变，以及心血管疾病（CVD）风险增加。本研究调查夜班工作是否会影响血糖变异性和失调，这可能是由昼夜节律失调所驱动的。该研究还将探讨这种干扰是否会升高参与动脉粥样硬化的炎症标志物，并导致CVD风险标志物的恶化。
该研究包括60名参与者：一家制药厂的轮班夜班工人（白班、中班和夜班）和日班工人（对照组）。我们将分三个阶段评估夜班工作对代谢和心血管健康的影响：初始的6周观察期（第一阶段）、CVD风险因素的基线登记（第二阶段）以及2年后CVD风险因素的随访评估（第三阶段）。第一阶段的登记包括从薪资数据中获取的工作时间、通过OURA手环（活动记录仪、体积描记法和体温）获取的睡眠指标、使用连续血糖监测仪对血糖的连续评估、自我报告的食物日记以及昼夜节律标志物（单核细胞mRNA表达）的测量。在第二阶段和第三阶段，测量血液中的CVD风险因素，如炎症标志物、血脂、糖化血红蛋白、D-二聚体、血压临床检查、静息心率、通过颈动脉到股动脉脉搏波速度测量动脉僵硬度、颈动脉内膜中层厚度和最大摄氧量（V̇O）。为此，将以前瞻性方式使用一套全面的方法，以提供关于轮班工作引起的血糖调节和CVD风险因素的新知识。
所有参与者在参与研究前均提供了书面知情同意书，该研究将遵循《赫尔辛基宣言》中概述的原则。挪威东南部B地区医学研究伦理委员会已批准伦理许可（参考编号# 745702）。传播计划包括学术和公众出版物，以及与国家和地区政策制定者的合作。
