Palakkan Anwar A, Nanda Jyoti, Ross James A
Tissue Injury and Repair Group, Chancellor's Building, University of Edinburgh, Edinburgh, UK.
Immunology & Stem Cell Biology, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India.
Methods Mol Biol. 2025;2924:31-43. doi: 10.1007/978-1-0716-4530-7_3.
We have developed a method to bulk up definitive endoderm cells generated from human iPSC, and can be further differentiated to hepatocytes. Human iPSC-derived definitive endoderm cells were sorted, based on the expression of CXCR4, and were able to proliferate for extended periods and can be cryopreserved. These cells were able to generate functional hepatocytes expressing albumin and alpha-fetoprotein in different multi-well formats. This method would help to produce more consistent hepatocytes and also enable the development of high throughput screening strategies.
我们已经开发出一种方法来扩增从人诱导多能干细胞（iPSC）生成的定形内胚层细胞，并且这些细胞能够进一步分化为肝细胞。基于CXCR4的表达对人iPSC衍生的定形内胚层细胞进行分选，这些细胞能够长期增殖并且可以冷冻保存。这些细胞能够在不同的多孔板形式中生成表达白蛋白和甲胎蛋白的功能性肝细胞。这种方法将有助于产生更一致的肝细胞，还能推动高通量筛选策略的发展。
