Palakkan Anwar A, Nanda Jyoti, Ross James A
Tissue Injury and Repair Group, Clinical Sciences, Edinburgh Medical School, University of Edinburgh, EH16 4SB Edinburgh, UK.
Biomed Rep. 2017 Apr;6(4):367-373. doi: 10.3892/br.2017.867. Epub 2017 Mar 1.
Over the past several years, there has been substantial progress in the field of regenerative medicine, which has enabled new possibilities for research and clinical application. For example, there are ongoing efforts directed at generating functional hepatocytes from adult-derived pluripotent cells for toxicity screening, generating disease models or, in the longer term, for the treatment of liver failure. In the present review, the authors summarise recent developments in regenerative medicine and pluripotent stem cells, the methods and tissues used for reprogramming and the differentiation of induced pluripotent stem cells (iPSCs) into hepatocyte-like cells. In addition, the hepatic disease models developed using iPSC technologies are discussed, as well as the potential for gene editing.
在过去几年中，再生医学领域取得了重大进展，为研究和临床应用带来了新的可能性。例如，目前正在努力从成人多能干细胞中生成功能性肝细胞，用于毒性筛查、建立疾病模型，或者从长远来看用于治疗肝衰竭。在本综述中，作者总结了再生医学和多能干细胞的最新进展、用于重编程的方法和组织，以及诱导多能干细胞（iPSC）向肝细胞样细胞的分化。此外，还讨论了使用iPSC技术建立的肝脏疾病模型以及基因编辑的潜力。
