Makhumbila Penny, Rauwane Molemi, Muedi Hangwani, Madala Ntakadzeni E, Figlan Sandiswa
Department of Agriculture and Animal Health, School of Agriculture and Life Sciences, College of Agriculture and Environmental Sciences, University of South Africa, 28 Pioneer Ave, Florida Park, Roodepoort, 1709, South Africa.
Department of Botany, Nelson Mandela University, South Campus, University Way, Summerstrand, Port Elizabeth, 6001, South Africa.
BMC Plant Biol. 2025 May 1;25(1):568. doi: 10.1186/s12870-025-06584-w.
Common bean (Phaseolus vulgaris L.) faces escalating challenges resulting from the increasing prevalence of fungal pathogens such as rust caused by Uromyces appendiculatus, threatening yields and quality of the crop. Understanding P. vulgaris' disease response mechanisms is pivotal for the crop's resilience and food security. Current scientific understanding of underlying molecular mechanisms of P. vulgaris to U. appendiculatus is limited, particularly with respect to specialised molecular data, including metabolite profiles and gene expression. There is a significant knowledge gap in explicating precise metabolomic and transcriptional changes that occur in P. vulgaris upon interaction with U. appendiculatus, which limits strategies aimed at enhancing pathogen resistance. In this study, biological stress response strategies of common bean to the rust pathogen were elucidated through a combined metabolomic and transcriptomic profiling approach. Our findings revealed that U. appendiculatus triggered diverse levels of 30 known metabolites, primarily flavonoids, lipids, nucleosides, and phenylpropanoids among others. Transcriptome sequencing detected over 3000 differentially expressed genes, including multiple transcription factor families such as heat shock proteins (HSPs), cytochrome P450 monooxygenases (CYP), terpene synthases and WRKY transcription factors (TFs) among others. Integrative metabolome and transcriptome analysis showed that rust infection enriched metabolomic pathways, biosynthesis of secondary metabolites, protein processing in the endoplasmic reticulum, and purine metabolism among others. The metabolome and transcriptome integration approach employed in this study provides insights on molecular mechanisms underlying U. appendiculatus response in P. vulgaris' key developmental stages.
普通菜豆（Phaseolus vulgaris L.）面临着越来越多的挑战，这些挑战源于真菌病原体（如由疣顶单胞锈菌引起的锈病）的流行率不断上升，威胁着作物的产量和质量。了解普通菜豆的病害反应机制对于作物的恢复力和粮食安全至关重要。目前，科学界对普通菜豆对疣顶单胞锈菌潜在分子机制的理解有限，特别是在包括代谢物谱和基因表达在内的专门分子数据方面。在解释普通菜豆与疣顶单胞锈菌相互作用时发生的精确代谢组学和转录变化方面存在重大知识差距，这限制了旨在增强病原体抗性的策略。在本研究中，通过联合代谢组学和转录组学分析方法，阐明了普通菜豆对锈病病原体的生物应激反应策略。我们的研究结果表明，疣顶单胞锈菌触发了30种已知代谢物的不同水平变化，主要包括黄酮类化合物、脂质、核苷和苯丙烷类化合物等。转录组测序检测到3000多个差异表达基因，包括多个转录因子家族，如热休克蛋白（HSPs）、细胞色素P450单加氧酶（CYP）、萜烯合酶和WRKY转录因子（TFs）等。代谢组和转录组的综合分析表明，锈病感染丰富了代谢组学途径、次生代谢物的生物合成、内质网中的蛋白质加工以及嘌呤代谢等。本研究采用的代谢组和转录组整合方法为普通菜豆关键发育阶段对疣顶单胞锈菌反应的分子机制提供了见解。
