Shimizu Nobutaka, Luo Fangjia, Tanaka Tomoyuki, Tono Kensuke, Yatabe Keiko, Iwata So, Nango Eriko
RIKEN SPring-8 Center, Sayo, Hyogo 679-5148, Japan.
Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki 305-0801, Japan.
Biophys Physicobiol. 2025 Mar 27;22(2):e220007. doi: 10.2142/biophysico.bppb-v22.0007. eCollection 2025.
A time-resolved small-angle X-ray scattering (SAXS) system for protein solution samples using an X-ray free-electron laser (XFEL) was established by developing a SAXS diffractometer by integrating a helium path into the DAPHNIS system initially designed for Serial Femtosecond Crystallography (SFX) experiments at BL2 of SACLA. This modification enabled us to successfully capture the SAXS profiles of ovalbumin under conditions without any reaction trigger, using both the newly developed system and the sample solution flow device that was originally designed for SFX experiments. Furthermore, we conducted acid denaturation experiments on cytochrome c, using a T-junction-type solution mixing flow system, and observed the denaturation-induced changes in the SAXS profiles.
通过在最初为SACLA的BL2的串行飞秒晶体学（SFX）实验设计的DAPHNIS系统中集成氦气路径来开发小角X射线散射（SAXS）衍射仪，建立了一种使用X射线自由电子激光（XFEL）的用于蛋白质溶液样品的时间分辨小角X射线散射（SAXS）系统。这种改进使我们能够使用新开发的系统和最初为SFX实验设计的样品溶液流动装置，在没有任何反应触发条件下成功捕获卵清蛋白的SAXS谱。此外，我们使用T型结型溶液混合流动系统对细胞色素c进行了酸变性实验，并观察了SAXS谱中变性引起的变化。
