Spring-8 相干光源（SACLA）中硬X射线衍射的多样应用平台（DAPHNIS）：在使用X射线自由电子激光的串行蛋白质晶体学中的应用
Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.
作者信息
Tono Kensuke, Nango Eriko, Sugahara Michihiro, Song Changyong, Park Jaehyun, Tanaka Tomoyuki, Tanaka Rie, Joti Yasumasa, Kameshima Takashi, Ono Shun, Hatsui Takaki, Mizohata Eiichi, Suzuki Mamoru, Shimamura Tatsuro, Tanaka Yoshiki, Iwata So, Yabashi Makina
机构信息
Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun 679-5198, Japan.
RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun 679-5148, Japan.
出版信息
J Synchrotron Radiat. 2015 May;22(3):532-7. doi: 10.1107/S1600577515004464. Epub 2015 Apr 16.
An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1 atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10 keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1 µm crystals at a resolution of 2.4 Å was obtained.
已开发出一种使用X射线自由电子激光（XFEL）进行串行飞秒晶体学研究的实验系统。它主要由一个样品腔、流体注射器和一个二维探测器组成。样品腔和注射器在1个大气压的氦气氛围下运行。常压操作便于应用于流体样品。采用三种注射器将随机取向的晶体注入水溶液或高粘性流体中。利用SPring-8埃紧凑型自由电子激光（SACLA）的10keV XFEL对溶菌酶晶体进行了实验。获得了分辨率为2.4Å的1μm晶体中模型蛋白溶菌酶的结构。
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