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产志贺毒素菌在婴儿结肠模型中诱导的微生物群和代谢组动力学。

Microbiota and metabolome dynamics induced by Shiga toxin-producing in an model of an infant's colon.

作者信息

Izquierdo Mariana, O'Sullivan Deborah, Uriot Ophélie, Brun Morgane, Durif Claude, Denis Sylvain, Gallardo Pablo, Gahan Cormac G M, Etienne-Mesmin Lucie, Blanquet-Diot Stéphanie, Farfan Mauricio J

机构信息

Departamento de Pediatría y Cirugía Infantil Oriente, CICA Hospital Dr. Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, 7500539 Santiago, Chile.

Equal contribution as a first author.

出版信息

Microb Cell. 2025 Apr 14;12:76-92. doi: 10.15698/mic2025.04.847. eCollection 2025.

DOI:10.15698/mic2025.04.847
PMID:40309356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12042126/
Abstract

Shiga toxin-producing Escherichia coli (STEC) is a major food-borne pathogen causing human diseases ranging from diarrhea to life-threatening complications, mainly in young children. Colonization, virulence, and interactions of STEC strains with human gut microbiota are pivotal during infection but remain poorly described, particularly in children, the most affected population. In this work, we evaluated changes in the microbiota and metabolome composition in the gut model: Toddler ARtificial COLon (T-ARCOL) infected with EHEC O157:H7 strain EDL 933. Stool samples collected from children with STEC-positive diarrhea and stool from the same children after recovery from the diarrheal episode () were used to inoculate the T-ARCOL model. STEC colonization was progressively reduced throughout fermentation in T-ARCOL with diarrhea or recovery fecal samples. Beta diversity showed that the diarrhea-associated microbiota was significantly distinct from the recovery microbiota and exhibited a lower α-diversity. In contrast to recovery conditions, diarrheal conditions were characterized by an increased abundance of potential pathobionts such as members of the family and higher acetate, succinate, and N-acetylneuraminic acid levels. Our results provide new evidence of the impact of EHEC in the microbiota and metabolome dynamics in an in vitro gut model that could be useful in understanding their physiopathology in this at-risk population, considering inter-individual variabilities in gut microbiota.

摘要

产志贺毒素大肠杆菌（STEC）是一种主要的食源性病原体，可导致人类从腹泻到危及生命的并发症等多种疾病，主要发生在幼儿中。STEC菌株在感染期间的定植、毒力以及与人类肠道微生物群的相互作用至关重要，但目前描述较少，尤其是在受影响最严重的儿童群体中。在这项研究中，我们评估了感染肠出血性大肠杆菌O157:H7菌株EDL 933的肠道模型：幼儿人工结肠（T-ARCOL）中微生物群和代谢组组成的变化。从STEC阳性腹泻儿童收集的粪便样本以及腹泻发作恢复后同一儿童的粪便样本用于接种T-ARCOL模型。在T-ARCOL中，使用腹泻或恢复粪便样本进行发酵的整个过程中，STEC的定植逐渐减少。β多样性表明，与腹泻相关的微生物群与恢复后的微生物群明显不同，且α多样性较低。与恢复状态相比，腹泻状态的特征是潜在致病共生菌（如某科成员）丰度增加，乙酸盐、琥珀酸盐和N-乙酰神经氨酸水平升高。我们的研究结果为肠出血性大肠杆菌对体外肠道模型中微生物群和代谢组动态的影响提供了新证据，考虑到肠道微生物群的个体间变异性，这可能有助于理解其在这一高危人群中的生理病理学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/c7e32d6cb7c5/mic-12-076-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/b346b317e55c/mic-12-076-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/80112bea20d3/mic-12-076-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/b9aa4e38a1c1/mic-12-076-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/a04ee4360278/mic-12-076-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bdd/12042126/c67fd2f6a41e/mic-12-076-g008.jpg
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A metagenomic approach to unveil the association between fecal gut microbiota and short-chain fatty acids in diarrhea caused by diarrheagenic in children.一种宏基因组学方法，用于揭示儿童致泻性大肠杆菌引起的腹泻中肠道粪便微生物群与短链脂肪酸之间的关联。
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