Chang T M, Erway B, Chey W Y
J Chromatogr. 1985 Jun 19;326:121-7. doi: 10.1016/s0021-9673(01)87437-9.
A rapid reversed-phase high-performance liquid chromatography method was developed for the isolation of small quantities of biologically active gastrointestinal hormones, using a Varian MCH-10 C18 column. Biologically active secretin was isolated from contamination with other hormones, including cholecystokinin, gastrin, motilin, and vasoactive intestinal polypeptide, from samples of the acid perfusate of canine duodenum and from the crude acetic acid extract of canine antral mucosa containing less than 100 picomoles of secretin. The method also appeared to be suitable for the isolation of cholecystokinin octapeptide and motilin.
开发了一种快速反相高效液相色谱法，用于分离少量生物活性胃肠激素，该方法使用Varian MCH-10 C18柱。从犬十二指肠酸灌注液样本以及含有少于100皮摩尔促胰液素的犬胃窦黏膜粗乙酸提取物中，将生物活性促胰液素与其他激素（包括胆囊收缩素、胃泌素、胃动素和血管活性肠肽）的污染分离出来。该方法似乎也适用于分离八肽胆囊收缩素和胃动素。
