Stec W J, Zon G, Uznanski B
J Chromatogr. 1985 Jun 19;326:263-80. doi: 10.1016/s0021-9673(01)87452-5.
Diastereomeric phosphorothioate analogues of oligodeoxyribonucleotides, which were synthesized by an automated, solid-phase, phosphoramidite-coupling method, were conveniently separated by reversed-phase high-performance liquid chromatography on a muBondapak C18 column with a gradient of acetonitrile in triethylammonium acetate buffer. These synthetic and chromatographic methods were also used to obtain diastereomerically pure bis-phosphorothioate, alkanephosphonate, and O-alkyl phosphotriester analogues of DNA for exploratory studies of stereochemistry and phosphorolytic enzymes.
通过自动固相亚磷酰胺偶联法合成的寡脱氧核糖核苷酸的非对映体硫代磷酸酯类似物，可方便地在μBondapak C18柱上，用乙腈在醋酸三乙铵缓冲液中的梯度进行反相高效液相色谱分离。这些合成和色谱方法还用于获得对映体纯的双硫代磷酸酯、链烷膦酸酯和DNA的O-烷基磷酸三酯类似物，用于立体化学和磷酸解酶的探索性研究。
