Ehrat M, Cecchini D J, Giese R W
J Chromatogr. 1985 Jun 19;326:311-20. doi: 10.1016/s0021-9673(01)87456-2.
Transamination conjugates of cytidine-3'-phosphate and polycytidylic acid (poly C) with diaminopropane, diaminohexane and diaminooctane (DAO) are formed both at 25 degrees C and 60 degrees C. The extent of reaction and formation of side products, with intermittent hydrolysis to mononucleotides in the case of aminoalkyl-poly C, is monitored by reversed-phase high-performance liquid chromatography. Both Dns-DAO-poly C and succinyl-lysozyme-DAO-poly C covalent conjugates are then prepared and similarly characterized, including separation on a size-exclusion diol high-performance liquid chromatography column. The retention of the latter on a wide-pore reversed-phase column seems to be controlled by the protein moiety.
胞苷-3'-磷酸和聚胞苷酸（聚C）与二氨基丙烷、二氨基己烷和二氨基辛烷（DAO）的转氨共轭物在25℃和60℃下均能形成。对于氨基烷基-聚C，反应程度和副产物的形成情况，以及间歇性水解为单核苷酸的情况，通过反相高效液相色谱法进行监测。然后制备了丹磺酰-DAO-聚C和琥珀酰-溶菌酶-DAO-聚C共价共轭物，并进行了类似的表征，包括在尺寸排阻二醇高效液相色谱柱上进行分离。后者在宽孔反相柱上的保留似乎受蛋白质部分的控制。
