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Protein A-coated glass beads. Universal support medium for high-performance immunoaffinity chromatography.

作者信息

Phillips T M, Queen W D, More N S, Thompson A M

出版信息

J Chromatogr. 1985 Jun 26;327:213-9. doi: 10.1016/s0021-9673(01)81651-4.

Abstract

High-performance immunoaffinity chromatography (HPIC) is a technique for the fast isolation and quantitation of both antibodies and antigens. Protein A-coated glass beads provide a stable general immobilization support for most immunoglobulin G (IgG) antibodies. In conjunction with the modern expanding repertoire of monoclonal antibodies, HPIC can be applied to the quantitation and isolation of any biological material, in an active form.

摘要

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