Phillips T M, Queen W D, More N S, Thompson A M
J Chromatogr. 1985 Jun 26;327:213-9. doi: 10.1016/s0021-9673(01)81651-4.
High-performance immunoaffinity chromatography (HPIC) is a technique for the fast isolation and quantitation of both antibodies and antigens. Protein A-coated glass beads provide a stable general immobilization support for most immunoglobulin G (IgG) antibodies. In conjunction with the modern expanding repertoire of monoclonal antibodies, HPIC can be applied to the quantitation and isolation of any biological material, in an active form.
高效免疫亲和色谱法(HPIC)是一种用于快速分离和定量抗体及抗原的技术。蛋白A包被的玻璃珠为大多数免疫球蛋白G(IgG)抗体提供了稳定的通用固定化支持。结合现代不断扩大的单克隆抗体库,HPIC可应用于以活性形式定量和分离任何生物材料。