Lantz Abigail E, Gordián Edna R, Rosa Marilin, Rodríguez-Ruíz Marileana, Johnson Joseph O, Gebert Ryan, Bahr Allison, Chen Dung Tsa, Dutil Julie, Li Jiannong, Oliveras Torres José A, Saavedra Harold I, Eschrich Steven A, Flores Idhaliz, Cress William D
Department of Molecular Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida.
Department of Pathology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida.
Cancer Res Commun. 2025 May 1;5(5):804-813. doi: 10.1158/2767-9764.CRC-24-0650.
Breast cancer is a leading cause of cancer-related mortality among women worldwide and is known to have higher mortality among women with African ancestry. Herein, we describe the creation and characterization of a multiethnic breast cancer tissue microarray (ME-BrTMA) representing tumors from non-Hispanic White (n = 41), non-Hispanic Black (NHB; n = 45), and Hispanic patients from Puerto Rico (n = 36) and Florida (n = 52). This ME-BrTMA comprises five blocks with a total of 610 cores: 371 breast cancer tumor cores, 93 breast stromal cores, 96 normal breast tissue cores, 30 non-breast cancer tumor cores, and 20 cores representing normal tissues. Initial characterization of the ME-BrTMA includes standard IHC staining of well-characterized clinical biomarkers, including the estrogen hormone receptors and progesterone hormone receptors, HER2, and Ki-67, interpreted by the coauthoring pathologist (Marilin Rosa). The IHC results indicated good but imperfect alignment with clinical diagnoses. Cores from breast cancer tumors from the NHB cohort most frequently scored negative for estrogen receptor (63%, P < 0.005) and progesterone receptor (80%, P < 0.005) and most frequently have high expression of the Ki-67 proliferation marker (38%, P < 0.05). Prediction Analysis of Microarray 50 (PAM50) analysis using RNA from secondary patient blocks showed that the NHB group also most frequently scored in the basal-like category (61%, P < 0.05). Taken together, the initial characterization of the ME-BrTMA suggests that it may serve as a representative resource to understand the underlying biology of breast cancer and its relationship to patient outcomes.
The ME-BrTMA described herein provides a resource that may serve as a tool to understand the underlying biology of breast cancer.
乳腺癌是全球女性癌症相关死亡的主要原因，并且已知在有非洲血统的女性中死亡率更高。在此，我们描述了一种多民族乳腺癌组织微阵列（ME-BrTMA）的创建和特征，该微阵列代表了来自非西班牙裔白人（n = 41）、非西班牙裔黑人（NHB；n = 45）以及来自波多黎各（n = 36）和佛罗里达（n = 52）的西班牙裔患者的肿瘤。这个ME-BrTMA由五个模块组成，共有610个芯块：371个乳腺癌肿瘤芯块、93个乳腺基质芯块、96个正常乳腺组织芯块、30个非乳腺癌肿瘤芯块以及20个代表正常组织的芯块。ME-BrTMA的初步特征包括对特征明确的临床生物标志物进行标准免疫组化染色，这些生物标志物包括雌激素受体和孕激素受体、HER2以及Ki-67，由共同撰写的病理学家（玛丽琳·罗萨）进行解读。免疫组化结果表明与临床诊断有较好但并不完美的一致性。NHB队列中乳腺癌肿瘤的芯块雌激素受体最常呈阴性（63%，P < 0.005），孕激素受体也最常呈阴性（80%，P < 0.005），并且Ki-67增殖标志物最常高表达（38%，P < 0.05）。使用来自二次患者模块的RNA进行的微阵列50（PAM50）预测分析表明，NHB组也最常被归类为基底样类别（61%，P < 0.05）。总体而言，ME-BrTMA的初步特征表明它可能作为一种代表性资源，用于理解乳腺癌的潜在生物学特性及其与患者预后的关系。
本文所述的ME-BrTMA提供了一种资源，可作为理解乳腺癌潜在生物学特性的工具。
