Kuriu Ayano, Ishikawa Kazuya, Tsuchiya Kohsuke, Furuta Kazuyuki, Kaito Chikara
Division of Molecular Biology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.
Infect Immun. 2025 Jun 10;93(6):e0012625. doi: 10.1128/iai.00126-25. Epub 2025 May 1.
Animal infection models are essential for understanding bacterial pathogenicity and corresponding host immune responses. In this study, we investigated whether juvenile could be used as an infection model for human pathogenic bacteria. frogs succumbed to intraperitoneal injection containing the human pathogenic bacteria , , and . In contrast, non-pathogenic bacteria and did not induce mortality in frogs. The administration of appropriate antibiotics suppressed mortality caused by and . Strains lacking the locus, () gene, or hemolysin genes in , LIPI-1-deleted mutant of , which attenuate virulence within mammals, exhibited reduced virulence in frogs compared with their respective wild-type counterparts. Bacterial distribution analysis revealed that persisted in the blood, liver, heart, and muscles of frogs until death. These results suggested that intraperitoneal injection of human pathogenic bacteria induces sepsis-like symptoms in frogs, supporting their use as a valuable animal model for evaluating antimicrobial efficacy and identifying virulence genes in various human pathogenic bacteria.
动物感染模型对于理解细菌致病性和相应的宿主免疫反应至关重要。在本研究中，我们调查了幼蛙是否可用作人类病原菌的感染模型。幼蛙在腹腔注射含人类病原菌、和后死亡。相比之下，非病原菌和未在幼蛙中诱导死亡。给予适当的抗生素可抑制由和引起的死亡。在哺乳动物体内毒力减弱的、缺失基因座、（）基因或溶血素基因的菌株，与各自的野生型对应菌株相比，在幼蛙中的毒力降低。细菌分布分析显示，在幼蛙的血液、肝脏、心脏和肌肉中持续存在直至死亡。这些结果表明，腹腔注射人类病原菌可在幼蛙中诱导类似败血症的症状，支持将其用作评估抗菌疗效和鉴定各种人类病原菌中毒力基因的有价值动物模型。
