从墨西哥华雷斯城一名患者身上分离出的登革热病毒1型的特性分析

Characterization of a Dengue Virus Serotype 1 Isolated from a Patient in Ciudad Juarez, Mexico.

作者信息

Palermo Pedro M, de la Mora-Covarrubias Antonio, Orbegozo Jeanette, Plante Jessica A, Plante Kenneth S, Jimenez-Vega Florinda, Watts Douglas M

机构信息

Department of Biological Sciences and Border Biomedical Research Center, University of Texas at El Paso, El Paso, TX 79968, USA.

Instituto de Ciencias Biomedicas, Universidad Autonoma de Ciudad Juarez, Juarez City 32100, Mexico.

出版信息

Pathogens. 2021 Jul 10;10(7):872. doi: 10.3390/pathogens10070872.

DOI:10.3390/pathogens10070872

PMID:34358022

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8308707/

Abstract

Dengue (DEN) is the most important human arboviral disease worldwide. Sporadic outbreaks of DEN have been reported since 1980 in urban communities located along the border in southeast Texas and northern Mexico. Other than the Rio Grande Valley region of TX, autochthonous transmission of DENV has not been reported from any other US border communities. As part of a surveillance program for arthropod-borne viruses in Ciudad Juarez, Mexico, during November 2015, a blood sample was obtained from a female patient who experienced an undifferentiated fever and arthralgia. The plasma of the sample was tested for virus in Vero-76 and C6/36 cells. DENV serotype 1 (DENV-1) was isolated in the C6/36 cells, and nucleotide sequencing of the envelope gene and full genome grouped the DENV-1 isolate in the Central America clade. The patient had not traveled outside of Ciudad Juarez, Mexico, thus suggesting DENV-1 infection was acquired in this community.

摘要

登革热（DEN）是全球最重要的人类虫媒病毒病。自1980年以来，在得克萨斯州东南部和墨西哥北部边境沿线的城市社区报告了登革热的零星疫情。除了得克萨斯州的里奥格兰德河谷地区外，美国其他边境社区均未报告过登革病毒的本土传播情况。作为墨西哥华雷斯城节肢动物传播病毒监测项目的一部分，2015年11月，从一名出现不明原因发热和关节痛的女性患者身上采集了一份血样。对该样本的血浆在Vero-76和C6/36细胞中进行病毒检测。在C6/36细胞中分离出登革病毒1型（DENV-1），包膜基因和全基因组的核苷酸测序将该DENV-1分离株归入中美洲分支。该患者未曾离开过墨西哥华雷斯城，因此表明该社区存在DENV-1感染。