Negrutiu Mircea, Danescu Sorina, Focsan Monica, Vesa Stefan Cristian, Cadar Adelina, Vaida Stefan, Oiegar Alexandra, Baican Adrian
Department of Dermatology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, 400084 Cluj-Napoca, Romania.
Diagnostics (Basel). 2025 Apr 16;15(8):1018. doi: 10.3390/diagnostics15081018.
: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer, with diverse clinical presentations. This study aims to correlate findings from dermoscopy, ultrasonography, ex vivo confocal microscopy, and histology to improve diagnostic accuracy and guide better clinical management of cSCC. : This cross-sectional study, conducted between July 2022 and December 2024, included 26 patients with 35 clinically suspicious cSCC tumors, analyzed through clinical, dermoscopic, high-frequency ultrasound (HFUS), ex vivo confocal fluorescence microscopy (FCM), and histopathology. Tumors were evaluated for various clinical, imaging, and histopathological criteria, such as tumor thickness, vascularization, differentiation degree, and invasion level, with FCM applied to 24 tumors for advanced microscopic analysis. : The study analyzed 35 cases of histopathologically confirmed cSCC, finding that invasive SCC was associated with greater tumor thickness, increased vascularization, and ulceration on both ultrasound and dermatoscopy, while in situ SCC showed homogeneous echogenicity and specific dermoscopic patterns like dotted vessels and white halos. Strong correlations were identified between ultrasound and histopathological measurements of tumor thickness and invasion depth, and confocal microscopy revealed that features like plump bright cells and nest-like structures were linked to invasive and poorly differentiated tumors. : This study uniquely integrates advanced imaging techniques-dermatoscopy, skin ultrasound, and ex vivo confocal microscopy-with histopathological analysis to provide new insights into tumor grade, vascularity, and invasion depth in cSCC, enhancing non-invasive diagnosis.
皮肤鳞状细胞癌（cSCC）是第二常见的皮肤癌，临床表现多样。本研究旨在关联皮肤镜检查、超声检查、离体共聚焦显微镜检查和组织学检查的结果，以提高诊断准确性并指导cSCC的更好临床管理。：这项横断面研究于2022年7月至2024年12月进行，纳入了26例患者的35个临床可疑cSCC肿瘤，通过临床、皮肤镜、高频超声（HFUS）、离体共聚焦荧光显微镜检查（FCM）和组织病理学进行分析。对肿瘤进行了各种临床、影像学和组织病理学标准的评估，如肿瘤厚度、血管形成、分化程度和浸润水平，其中24个肿瘤应用FCM进行高级显微镜分析。：该研究分析了35例组织病理学确诊的cSCC病例，发现浸润性SCC与更大的肿瘤厚度、超声和皮肤镜检查中血管形成增加及溃疡有关，而原位SCC表现为均匀的回声性和点状血管及白色晕圈等特定的皮肤镜模式。在超声与肿瘤厚度和浸润深度的组织病理学测量之间发现了强相关性，共聚焦显微镜检查显示丰满明亮细胞和巢状结构等特征与浸润性和低分化肿瘤有关。：本研究独特地将先进的成像技术——皮肤镜检查、皮肤超声和离体共聚焦显微镜检查——与组织病理学分析相结合，为cSCC的肿瘤分级、血管形成和浸润深度提供了新的见解，增强了非侵入性诊断。
