Qiu Cunpu, Bao Ying, Lü Dingding, Yan Mengyuan, Li Guilong, Liu Kai, Wei Shiping, Wu Meng, Li Zhongpei
Zhenjiang College, Zhenjiang, China.
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
Front Microbiol. 2025 Apr 17;16:1574765. doi: 10.3389/fmicb.2025.1574765. eCollection 2025.
Understanding the effects of bio-antimicrobial materials on plant growth and against diseases and the relevant mechanisms are highly important for sustainable soil use and plant safety production. This study explored the impacts and corresponding mechanisms of the combined utilization of humic acid, chitosan, and () on tomato growth and diseases occurrence through a greenhouse pot experiment. The plant height, fresh weight, disease index, rhizosphere microbial community, and root exudates composition of tomatoes were determined. With the combined application of humic acid, chitosan and (HBC), the height and fresh weight of tomato plants were significantly increased ( < 0.05), and the incidence of plant diseases was decreased by 45.1%. In HBC treatment, the diversity of fungal and bacterial communities was notably enhanced. The relative abundances of increased, while the relative abundances of especially soil-borne plant pathogen and significantly decreased ( < 0.05). Besides the increased diversity of root exudates, the content of phenolic acids, which are allelochemicals related to continuous cropping disorder, decreased. The results of cooccurrence network analysis indicated that the abundances of Eicosanoids, Fatty acids and conjugates, and Flavonoid lycosides compounds in root exudates, which are positively correlated with pathogenic bacteria, decreased in HBC treatment. Results indicated HBC's synergistic effect on tomato growth and disease resistance is related to its regulation of microbial community and root exudates. The study results promote the development of biological control technology and highlight its promising application in plant safety production.
了解生物抗菌材料对植物生长和抗病性的影响及其相关机制，对于土壤可持续利用和植物安全生产至关重要。本研究通过温室盆栽试验，探讨了腐殖酸、壳聚糖和（）联合使用对番茄生长和病害发生的影响及相应机制。测定了番茄的株高、鲜重、病情指数、根际微生物群落和根系分泌物组成。腐殖酸、壳聚糖和（HBC）联合施用后，番茄植株的株高和鲜重显著增加（<0.05），植物病害发生率降低了45.1%。在HBC处理中，真菌和细菌群落的多样性显著增强。的相对丰度增加，而尤其是土传植物病原菌和的相对丰度显著降低（<0.05）。除了根系分泌物多样性增加外，与连作障碍相关的化感物质酚酸含量降低。共现网络分析结果表明，HBC处理中根系分泌物中与病原菌呈正相关的类二十烷酸、脂肪酸及其共轭物和黄酮糖苷化合物的丰度降低。结果表明，HBC对番茄生长和抗病性的协同作用与其对微生物群落和根系分泌物的调节有关。研究结果促进了生物防治技术的发展，并突出了其在植物安全生产中的应用前景。
